Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins

Marcel Stoop, Lennard Dekker, Mark Titulaer, RJAN Lamers, Peter Burgers, Peter Sillevis Smitt, AJ (Alain) van Gool, Theo Luider, Rogier Hintzen

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Abstract

We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).
Original languageUndefined/Unknown
Pages (from-to)1404-1414
Number of pages11
JournalJournal of Proteome Research
Volume8
Issue number3
DOIs
Publication statusPublished - 2009

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