Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins

Marcel Stoop; Lennard Dekker; Mark Titulaer; RJAN Lamers; Peter Burgers; Peter Sillevis Smitt; AJ (Alain) van Gool; Theo Luider; Rogier Hintzen

doi:10.1021/pr8010155

Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins

Marcel Stoop, Lennard Dekker, Mark Titulaer, RJAN Lamers, Peter Burgers, Peter Sillevis Smitt, AJ (Alain) van Gool, Theo Luider, Rogier Hintzen

Research output: Contribution to journal › Article › Academic › peer-review

50 Citations (Scopus)

Abstract

We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).

Original language	Undefined/Unknown
Pages (from-to)	1404-1414
Number of pages	11
Journal	Journal of Proteome Research
Volume	8
Issue number	3
DOIs	https://doi.org/10.1021/pr8010155
Publication status	Published - 2009

Access to Document

10.1021/pr8010155

http://hdl.handle.net/1765/16460

Cite this

Stoop, M., Dekker, L., Titulaer, M., Lamers, RJAN., Burgers, P., Sillevis Smitt, P., van Gool, AJ., Luider, T., & Hintzen, R. (2009). Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins. Journal of Proteome Research, 8(3), 1404-1414. https://doi.org/10.1021/pr8010155

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title = "Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins",

abstract = "We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).",

author = "Marcel Stoop and Lennard Dekker and Mark Titulaer and RJAN Lamers and Peter Burgers and {Sillevis Smitt}, Peter and {van Gool}, {AJ (Alain)} and Theo Luider and Rogier Hintzen",

year = "2009",

doi = "10.1021/pr8010155",

language = "Undefined/Unknown",

volume = "8",

pages = "1404--1414",

journal = "Journal of Proteome Research",

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publisher = "American Chemical Society",

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Stoop, M, Dekker, L, Titulaer, M, Lamers, RJAN, Burgers, P , Sillevis Smitt, P, van Gool, AJ, Luider, T & Hintzen, R 2009, 'Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins', Journal of Proteome Research, vol. 8, no. 3, pp. 1404-1414. https://doi.org/10.1021/pr8010155

Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins. / Stoop, Marcel; Dekker, Lennard; Titulaer, Mark et al.

In: Journal of Proteome Research, Vol. 8, No. 3, 2009, p. 1404-1414.

Research output: Contribution to journal › Article › Academic › peer-review

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T1 - Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins

AU - Stoop, Marcel

AU - Dekker, Lennard

AU - Titulaer, Mark

AU - Lamers, RJAN

AU - Burgers, Peter

AU - Sillevis Smitt, Peter

AU - van Gool, AJ (Alain)

AU - Luider, Theo

AU - Hintzen, Rogier

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N2 - We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).

AB - We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).

U2 - 10.1021/pr8010155

DO - 10.1021/pr8010155

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JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

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