Raman spectroscopy for guidance of vulvar cancer surgery: A pilot study

Helena C. van Doorn; Elisa M.  Barroso; Senada Koljenović,; Patricia C.  Ewing-Graham; M. Rosa N.  Soares; Nick J.  van de Berg; Tom C.  Bakker Schut; Gerwin J.  Puppels

doi:10.1364/BOE.420882

Raman spectroscopy for guidance of vulvar cancer surgery: A pilot study

Helena C. van Doorn, Elisa M. Barroso, Senada Koljenović,, Patricia C. Ewing-Graham, M. Rosa N. Soares, Nick J. van de Berg^*, Tom C. Bakker Schut, Gerwin J. Puppels

^*Corresponding author for this work

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Abstract

For vulvar squamous cell carcinoma (VSCC), the mainstay of treatment is surgical removal with tumour-free margins. Surgeons still operate without objective tools that provide margin-status. This study assesses Raman spectroscopy potentiality for distinguishing ex-vivo VSCC from healthy tissue in 11 patients. Grid-based Raman maps were obtained from processed spectra. Water content and C-H band ratio (2,910-2,966 cm-1 / 2810-2890 cm-1) were calculated per spectrum and used as linear discriminant parameters. Healthy tissue was differentiated from VSCC with 0.90 discriminative power, 0.79 sensitivity and 0.86 specificity.This is an important step towards the development of objective tools for VSCC surgical guidance.

Original language	English
Pages (from-to)	3008-3020
Number of pages	13
Journal	Biomedical Optics Express
Volume	12
Issue number	5
DOIs	https://doi.org/10.1364/BOE.420882
Publication status	Published - 1 May 2021

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Raman spectroscopy for guidance of vulvar cancer surgery, a pilot studyFinal published version, 3.88 MBLicence: CC BY

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title = "Raman spectroscopy for guidance of vulvar cancer surgery: A pilot study",

abstract = "For vulvar squamous cell carcinoma (VSCC), the mainstay of treatment is surgical removal with tumour-free margins. Surgeons still operate without objective tools that provide margin-status. This study assesses Raman spectroscopy potentiality for distinguishing ex-vivo VSCC from healthy tissue in 11 patients. Grid-based Raman maps were obtained from processed spectra. Water content and C-H band ratio (2,910-2,966 cm-1 / 2810-2890 cm-1) were calculated per spectrum and used as linear discriminant parameters. Healthy tissue was differentiated from VSCC with 0.90 discriminative power, 0.79 sensitivity and 0.86 specificity.This is an important step towards the development of objective tools for VSCC surgical guidance. ",

author = "{van Doorn}, {Helena C.} and Barroso, {Elisa M.} and Senada Koljenovi{\'c}, and Ewing-Graham, {Patricia C.} and Soares, {M. Rosa N.} and {van de Berg}, {Nick J.} and {Bakker Schut}, {Tom C.} and Puppels, {Gerwin J.}",

note = "Publisher Copyright: {\textcopyright} 2021 Optical Society of America.",

year = "2021",

month = may,

day = "1",

doi = "10.1364/BOE.420882",

language = "English",

volume = "12",

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TY - JOUR

T1 - Raman spectroscopy for guidance of vulvar cancer surgery

T2 - A pilot study

AU - van Doorn, Helena C.

AU - Barroso, Elisa M.

AU - Koljenović,, Senada

AU - Ewing-Graham, Patricia C.

AU - Soares, M. Rosa N.

AU - van de Berg, Nick J.

AU - Bakker Schut, Tom C.

AU - Puppels, Gerwin J.

PY - 2021/5/1

Y1 - 2021/5/1

N2 - For vulvar squamous cell carcinoma (VSCC), the mainstay of treatment is surgical removal with tumour-free margins. Surgeons still operate without objective tools that provide margin-status. This study assesses Raman spectroscopy potentiality for distinguishing ex-vivo VSCC from healthy tissue in 11 patients. Grid-based Raman maps were obtained from processed spectra. Water content and C-H band ratio (2,910-2,966 cm-1 / 2810-2890 cm-1) were calculated per spectrum and used as linear discriminant parameters. Healthy tissue was differentiated from VSCC with 0.90 discriminative power, 0.79 sensitivity and 0.86 specificity.This is an important step towards the development of objective tools for VSCC surgical guidance.

AB - For vulvar squamous cell carcinoma (VSCC), the mainstay of treatment is surgical removal with tumour-free margins. Surgeons still operate without objective tools that provide margin-status. This study assesses Raman spectroscopy potentiality for distinguishing ex-vivo VSCC from healthy tissue in 11 patients. Grid-based Raman maps were obtained from processed spectra. Water content and C-H band ratio (2,910-2,966 cm-1 / 2810-2890 cm-1) were calculated per spectrum and used as linear discriminant parameters. Healthy tissue was differentiated from VSCC with 0.90 discriminative power, 0.79 sensitivity and 0.86 specificity.This is an important step towards the development of objective tools for VSCC surgical guidance.

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JO - Biomedical Optics Express

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ER -

Raman spectroscopy for guidance of vulvar cancer surgery: A pilot study

Abstract

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