Raman spectroscopy to discriminate laryngeal squamous cell carcinoma from non-cancerous surrounding tissue

Cornelia van Lanschot*, Tom Bakker Schut, Elisa Barroso, Aniel Sewnaik, Jose Hardillo, Dominiek Monserez, Cees Meeuwis, Stijn Keereweer, Rob Baatenburg de Jong, Gerwin Puppels, Senada Koljenović

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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As for many solid cancers, laryngeal cancer is treated surgically, and adequate resection margins are critical for survival. Raman spectroscopy has the capacity to accurately differentiate between cancer and non-cancerous tissue based on their molecular composition, which has been proven in previous work. The aim of this study is to investigate whether Raman spectroscopy can be used to discriminate laryngeal cancer from surrounding non-cancerous tissue. Patients surgically treated for laryngeal cancer were included. Raman mapping experiments were performed ex vivo on resection specimens and correlated to histopathology. Water concentration analysis and CH-stretching region analysis were performed in the high wavenumber range of 2500–4000 cm−1. Thirty-four mapping experiments on 22 resection specimens were used for analysis. Both laryngeal cancer and all non-cancerous tissue structures showed high water concentrations of around 75%. Discriminative information was only found to be present in the CH-stretching region of the Raman spectra of the larynx (discriminative power of 0.87). High wavenumber region Raman spectroscopy can discriminate laryngeal cancer from non-cancerous tissue structures. Contrary to the findings for oral cavity cancer, water concentration is not a discriminating factor for laryngeal cancer.

Original languageEnglish
Article number193
JournalLasers in Medical Science
Issue number1
Publication statusE-pub ahead of print - 25 Aug 2023

Bibliographical note

Funding Information:
This work was supported by Atos Medical B.V.

Publisher Copyright: © 2023, The Author(s).


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