Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis

Ingrid M E Desar; Maaike de Boer; Corina C P M Bens; Jan A Jacobs; Johan W Mouton; Anton S M Dofferhoff; Corné H W Klaassen

doi:10.1016/j.mimet.2008.06.022

Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis

Ingrid M E Desar, Maaike de Boer, Corina C P M Bens, Jan A Jacobs, Johan W Mouton, Anton S M Dofferhoff, Corné H W Klaassen

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

A real-time PCR assay is described for rapid and accurate identification of the Streptococcus anginosus group members. Based on melting curve analysis, the S. anginosus species isolates could be further subdivided into two subgroups, each associated with different clinically relevant ribotypes of S. anginosus.

Original language	English
Pages (from-to)	372-4
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	75
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2008.06.022
Publication status	Published - Oct 2008
Externally published	Yes

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10.1016/j.mimet.2008.06.022

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title = "Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis",

abstract = "A real-time PCR assay is described for rapid and accurate identification of the Streptococcus anginosus group members. Based on melting curve analysis, the S. anginosus species isolates could be further subdivided into two subgroups, each associated with different clinically relevant ribotypes of S. anginosus.",

author = "Desar, {Ingrid M E} and {de Boer}, Maaike and Bens, {Corina C P M} and Jacobs, {Jan A} and Mouton, {Johan W} and Dofferhoff, {Anton S M} and Klaassen, {Corn{\'e} H W}",

year = "2008",

month = oct,

doi = "10.1016/j.mimet.2008.06.022",

language = "English",

volume = "75",

pages = "372--4",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

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TY - JOUR

T1 - Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis

AU - Desar, Ingrid M E

AU - de Boer, Maaike

AU - Bens, Corina C P M

AU - Jacobs, Jan A

AU - Mouton, Johan W

AU - Dofferhoff, Anton S M

AU - Klaassen, Corné H W

PY - 2008/10

Y1 - 2008/10

N2 - A real-time PCR assay is described for rapid and accurate identification of the Streptococcus anginosus group members. Based on melting curve analysis, the S. anginosus species isolates could be further subdivided into two subgroups, each associated with different clinically relevant ribotypes of S. anginosus.

AB - A real-time PCR assay is described for rapid and accurate identification of the Streptococcus anginosus group members. Based on melting curve analysis, the S. anginosus species isolates could be further subdivided into two subgroups, each associated with different clinically relevant ribotypes of S. anginosus.

U2 - 10.1016/j.mimet.2008.06.022

DO - 10.1016/j.mimet.2008.06.022

M3 - Article

C2 - 18652853

SN - 0167-7012

VL - 75

SP - 372

EP - 374

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis

Abstract

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