Rapid and reliable real-time PCR assay for detection of the macrolide efflux gene and subsequent discrimination between its distinct subclasses mef(A) and mef(E)

Debby M Klomberg; Hanneke A de Valk; Johan W Mouton; Corné H W Klaassen

doi:10.1016/j.mimet.2004.10.004

Rapid and reliable real-time PCR assay for detection of the macrolide efflux gene and subsequent discrimination between its distinct subclasses mef(A) and mef(E)

Debby M Klomberg, Hanneke A de Valk, Johan W Mouton, Corné H W Klaassen

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

A real-time PCR assay is described for detection of the macrolide efflux gene, mef. Following amplification, unambiguous discrimination between the two mef subclasses, mef(A) and mef(E), is easily established using a melting curve analysis. The results of this novel assay were 100% concordant with a conventional PCR-RFLP approach but requires far less hands-on time. Furthermore, the real-time format offers semiquantitative results allowing identification of contaminated cultures and/or DNA preparations.

Original language	English
Pages (from-to)	269-73
Number of pages	5
Journal	Journal of Microbiological Methods
Volume	60
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2004.10.004
Publication status	Published - Feb 2005
Externally published	Yes

Access to Document

10.1016/j.mimet.2004.10.004

Cite this

@article{d233b43314cc48c081fcc465eff1eed3,

title = "Rapid and reliable real-time PCR assay for detection of the macrolide efflux gene and subsequent discrimination between its distinct subclasses mef(A) and mef(E)",

abstract = "A real-time PCR assay is described for detection of the macrolide efflux gene, mef. Following amplification, unambiguous discrimination between the two mef subclasses, mef(A) and mef(E), is easily established using a melting curve analysis. The results of this novel assay were 100% concordant with a conventional PCR-RFLP approach but requires far less hands-on time. Furthermore, the real-time format offers semiquantitative results allowing identification of contaminated cultures and/or DNA preparations.",

author = "Klomberg, {Debby M} and {de Valk}, {Hanneke A} and Mouton, {Johan W} and Klaassen, {Corn{\'e} H W}",

year = "2005",

month = feb,

doi = "10.1016/j.mimet.2004.10.004",

language = "English",

volume = "60",

pages = "269--73",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "2",

}

Rapid and reliable real-time PCR assay for detection of the macrolide efflux gene and subsequent discrimination between its distinct subclasses mef(A) and mef(E). / Klomberg, Debby M; de Valk, Hanneke A; Mouton, Johan W et al.
In: Journal of Microbiological Methods, Vol. 60, No. 2, 02.2005, p. 269-73.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Rapid and reliable real-time PCR assay for detection of the macrolide efflux gene and subsequent discrimination between its distinct subclasses mef(A) and mef(E)

AU - Klomberg, Debby M

AU - de Valk, Hanneke A

AU - Mouton, Johan W

AU - Klaassen, Corné H W

PY - 2005/2

Y1 - 2005/2

N2 - A real-time PCR assay is described for detection of the macrolide efflux gene, mef. Following amplification, unambiguous discrimination between the two mef subclasses, mef(A) and mef(E), is easily established using a melting curve analysis. The results of this novel assay were 100% concordant with a conventional PCR-RFLP approach but requires far less hands-on time. Furthermore, the real-time format offers semiquantitative results allowing identification of contaminated cultures and/or DNA preparations.

AB - A real-time PCR assay is described for detection of the macrolide efflux gene, mef. Following amplification, unambiguous discrimination between the two mef subclasses, mef(A) and mef(E), is easily established using a melting curve analysis. The results of this novel assay were 100% concordant with a conventional PCR-RFLP approach but requires far less hands-on time. Furthermore, the real-time format offers semiquantitative results allowing identification of contaminated cultures and/or DNA preparations.

U2 - 10.1016/j.mimet.2004.10.004

DO - 10.1016/j.mimet.2004.10.004

M3 - Article

C2 - 15590101

SN - 0167-7012

VL - 60

SP - 269

EP - 273

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

Rapid and reliable real-time PCR assay for detection of the macrolide efflux gene and subsequent discrimination between its distinct subclasses mef(A) and mef(E)

Abstract

Access to Document

Fingerprint

Cite this