Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

FX López-Labrador; JR Brown; N Fischer; H Harvala; Sander van Boheemen; O Cinek; A Sayiner; TV Madsen; E Auvinen; V Kufner; M Huber; C Rodriguez; M Jonges; M Hönemann; P Susi; H Sousa; PE Klapper; A Pérez-Catalu?a; M Hernandez; Richard Molenkamp; LV der Hoek; R (Rob) Schuurman; N Couto; K Leuzinger; P Simmonds; M Beer; D Höper; S Kamminga; MCW Feltkamp; J Rodríguez-Díaz; E Keyaerts; XC Nielsen; E Puchhammer-Stöckl; AC Kroes; J Buesa; J Breuer; ECJ Claas; J de Vries

doi:10.1016/j.jcv.2020.104691

Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

FX López-Labrador, JR Brown, N Fischer, H Harvala, Sander van Boheemen, O Cinek, A Sayiner, TV Madsen, E Auvinen, V Kufner, M Huber, C Rodriguez, M Jonges, M Hönemann, P Susi, H Sousa, PE Klapper, A Pérez-Catalu?a, M Hernandez, Richard MolenkampLV der Hoek, R (Rob) Schuurman, N Couto, K Leuzinger, P Simmonds, M Beer, D Höper, S Kamminga, MCW Feltkamp, J Rodríguez-Díaz, E Keyaerts, XC Nielsen, E Puchhammer-Stöckl, AC Kroes, J Buesa, J Breuer, ECJ Claas, J de Vries

Virology

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Abstract

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

Original language	English
Article number	104691
Journal	Journal of Clinical Virology
Volume	134
DOIs	https://doi.org/10.1016/j.jcv.2020.104691
Publication status	Published - Jan 2021

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Publisher Copyright:
© 2020 The Authors

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López-Labrador, FX., Brown, JR., Fischer, N., Harvala, H., van Boheemen, S., Cinek, O., Sayiner, A., Madsen, TV., Auvinen, E., Kufner, V., Huber, M., Rodriguez, C., Jonges, M., Hönemann, M., Susi, P., Sousa, H., Klapper, PE., Pérez-Catalu?a, A., Hernandez, M., ... de Vries, J. (2021). Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure. Journal of Clinical Virology, 134, Article 104691. https://doi.org/10.1016/j.jcv.2020.104691

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title = "Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure",

abstract = "Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.",

author = "FX L{\'o}pez-Labrador and JR Brown and N Fischer and H Harvala and {van Boheemen}, Sander and O Cinek and A Sayiner and TV Madsen and E Auvinen and V Kufner and M Huber and C Rodriguez and M Jonges and M H{\"o}nemann and P Susi and H Sousa and PE Klapper and A P{\'e}rez-Catalu?a and M Hernandez and Richard Molenkamp and {der Hoek}, LV and Schuurman, {R (Rob)} and N Couto and K Leuzinger and P Simmonds and M Beer and D H{\"o}per and S Kamminga and MCW Feltkamp and J Rodr{\'i}guez-D{\'i}az and E Keyaerts and XC Nielsen and E Puchhammer-St{\"o}ckl and AC Kroes and J Buesa and J Breuer and ECJ Claas and {de Vries}, J",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2021",

month = jan,

doi = "10.1016/j.jcv.2020.104691",

language = "English",

volume = "134",

journal = "Journal of Clinical Virology",

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López-Labrador, FX, Brown, JR, Fischer, N, Harvala, H, van Boheemen, S, Cinek, O, Sayiner, A, Madsen, TV, Auvinen, E, Kufner, V, Huber, M, Rodriguez, C, Jonges, M, Hönemann, M, Susi, P, Sousa, H, Klapper, PE, Pérez-Catalu?a, A, Hernandez, M, Molenkamp, R, der Hoek, LV, Schuurman, R, Couto, N, Leuzinger, K, Simmonds, P, Beer, M, Höper, D, Kamminga, S, Feltkamp, MCW, Rodríguez-Díaz, J, Keyaerts, E, Nielsen, XC, Puchhammer-Stöckl, E, Kroes, AC, Buesa, J, Breuer, J, Claas, ECJ & de Vries, J 2021, 'Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure', Journal of Clinical Virology, vol. 134, 104691. https://doi.org/10.1016/j.jcv.2020.104691

TY - JOUR

T1 - Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

AU - López-Labrador, FX

AU - Brown, JR

AU - Fischer, N

AU - Harvala, H

AU - van Boheemen, Sander

AU - Cinek, O

AU - Sayiner, A

AU - Madsen, TV

AU - Auvinen, E

AU - Kufner, V

AU - Huber, M

AU - Rodriguez, C

AU - Jonges, M

AU - Hönemann, M

AU - Susi, P

AU - Sousa, H

AU - Klapper, PE

AU - Pérez-Catalu?a, A

AU - Hernandez, M

AU - Molenkamp, Richard

AU - der Hoek, LV

AU - Schuurman, R (Rob)

AU - Couto, N

AU - Leuzinger, K

AU - Simmonds, P

AU - Beer, M

AU - Höper, D

AU - Kamminga, S

AU - Feltkamp, MCW

AU - Rodríguez-Díaz, J

AU - Keyaerts, E

AU - Nielsen, XC

AU - Puchhammer-Stöckl, E

AU - Kroes, AC

AU - Buesa, J

AU - Breuer, J

AU - Claas, ECJ

AU - de Vries, J

PY - 2021/1

Y1 - 2021/1

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AB - Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

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U2 - 10.1016/j.jcv.2020.104691

DO - 10.1016/j.jcv.2020.104691

M3 - Article

SN - 1386-6532

VL - 134

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

M1 - 104691

ER -

Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

Abstract

Bibliographical note

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