TY - JOUR
T1 - Recommendations for the introduction of metagenomic next-generation sequencing in clinical virology, part II
T2 - bioinformatic analysis and reporting
AU - de Vries, Jutte J.C.
AU - Brown, Julianne R.
AU - Couto, Natacha
AU - Beer, Martin
AU - Le Mercier, Philippe
AU - Sidorov, Igor
AU - Papa, Anna
AU - Fischer, Nicole
AU - Oude Munnink, Bas B.
AU - Rodriquez, Christophe
AU - Zaheri, Maryam
AU - Sayiner, Arzu
AU - Hönemann, Mario
AU - Cataluna, Alba Perez
AU - Carbo, Ellen C.
AU - Bachofen, Claudia
AU - Kubacki, Jakub
AU - Schmitz, Dennis
AU - Tsioka, Katerina
AU - Matamoros, Sébastien
AU - Höper, Dirk
AU - Hernandez, Marta
AU - Puchhammer-Stöckl, Elisabeth
AU - Lebrand, Aitana
AU - Huber, Michael
AU - Simmonds, Peter
AU - Claas, Eric C.J.
AU - López-Labrador, F. Xavier
N1 - Publisher Copyright:
© 2021
PY - 2021/5
Y1 - 2021/5
N2 - Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety of sample types from patients with infectious syndromes. mNGS is still in its early stages of broader translation into clinical applications. To further support the development, implementation, optimization and standardization of mNGS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mNGS for viral diagnostics to share methodologies and experiences, and to develop application guidelines. Following the ENNGS publication Recommendations for the introduction of mNGS in clinical virology, part I: wet lab procedure in this journal, the current manuscript aims to provide practical recommendations for the bioinformatic analysis of mNGS data and reporting of results to clinicians.
AB - Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety of sample types from patients with infectious syndromes. mNGS is still in its early stages of broader translation into clinical applications. To further support the development, implementation, optimization and standardization of mNGS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mNGS for viral diagnostics to share methodologies and experiences, and to develop application guidelines. Following the ENNGS publication Recommendations for the introduction of mNGS in clinical virology, part I: wet lab procedure in this journal, the current manuscript aims to provide practical recommendations for the bioinformatic analysis of mNGS data and reporting of results to clinicians.
UR - http://www.scopus.com/inward/record.url?scp=85103629761&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2021.104812
DO - 10.1016/j.jcv.2021.104812
M3 - Article
C2 - 33819811
AN - SCOPUS:85103629761
SN - 1386-6532
VL - 138
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
M1 - 104812
ER -
