Recycling glycoproteins do not return to the cis-Golgi

J. J. Neefjes, J. M.H. Verkerk, H. J.G. Broxterman, G. A. Van Der Marel, J. H. Van Boom, H. L. Ploegh

Research output: Contribution to journalArticleAcademicpeer-review

44 Citations (Scopus)
3 Downloads (Pure)

Abstract

Recycling of a number of glycoproteins along the site of action of mannosidase I (the distal endoplasmic reticulum/cis-Golgi) was followed in several different cell lines. Treatment of cells with 1-deoxymannojirimycin (dMM) produced high mannose oligosaccharides at positions otherwise occupied by complex-type oligosaccharides in these glycoproteins. Conversion of high-mannose-type oligosaccharides to complex oligosaccharides of proteins initially synthesized in the presence of dMM was used as a marker for recycling of glycoproteins along the site of action of dMM. In contrast to findings reported by Snider and Rogers (Snider, M. D., and O. C. Rogers. 1986. J. Cell Biol. 103:265-275), removal of dMM did not result in reconversion of high-mannose oligosaccharides to complex-type sugars, even after prolonged periods of culture. We conclude that surface glycoproteins do not recycle through the cis-medial Golgi elements.

Original languageEnglish
Pages (from-to)79-87
Number of pages9
JournalJournal of Cell Biology
Volume107
Issue number1
DOIs
Publication statusPublished - 1 Jul 1988
Externally publishedYes

Bibliographical note

© The Rockefeller University Press

Fingerprint

Dive into the research topics of 'Recycling glycoproteins do not return to the cis-Golgi'. Together they form a unique fingerprint.

Cite this