Renin and prorenin have no direct effect on aldosterone synthesis in the human adrenocortical cell lines H295R and HAC15

Pieter Jansen, Hans Hofland, Ton van den Meiracker, Frank Jong, Jan Danser

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Introduction: Transgenic rats expressing the human (pro) renin receptor (h(P)RR) have elevated plasma aldosterone levels despite unaltered levels, in plasma and adrenal, of renin and angiotensin II. Materials and methods: To investigate whether renin/prorenin-(P) RR interaction underlies these elevated aldosterone levels, the effect of (pro) renin on steroidogenesis was compared with that of angiotensin II in two (P) RR-expressing human adrenocortical cell lines, H295R and HAC15. Angiotensin II rapidly induced extracellular signal-regulated kinase (ERK) phosphorylation and increased the expression of STAR, CYP21A2, CYP11B2, and CYP17A1 at 6 and 24 hours, whereas the expression of CYP11A1 an Conclusion: Although the (P) RR is present in adrenocortical cells, renin and prorenin do not elicit ERK phosphorylation nor directly affect steroid production via this receptor at nanomolar concentrations. Thus, direct (pro) renin-(P) RR interaction is unlikely to contribute to the elevated aldosterone levels in human (P) RR transgenic rats. This conclusion also implies that the aldosterone rise that often occurs during prolonged renin-angiotensin system blockade is rather due to the angiotensi
Original languageUndefined/Unknown
Pages (from-to)360-366
Number of pages7
JournalJournal of the Renin-Angiotensin-Aldosterone System
Volume13
Issue number3
DOIs
Publication statusPublished - 2012

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