TY - JOUR
T1 - Repository of Eurasian influenza A virus hemagglutinin and neuraminidase reverse genetics vectors and recombinant viruses
AU - Keawcharoen, Juthatip
AU - Spronken, Monique
AU - Vuong, Oanh
AU - Bestebroer, Theo
AU - Munster, VJ (Vincent)
AU - Osterhaus, Ab
AU - Rimmelzwaan, Guus
AU - Fouchier, Ron
PY - 2010
Y1 - 2010
N2 - Reverse genetics can be used to produce recombinant influenza A viruses containing virtually every desired combination of hemagglutinin (HA) and neuraminidase (NA) genes using the virus backbone of choice. Here, a repository of plasmids and recombinant viruses representing all contemporary Eurasian HA and NA subtypes, H1-H16 and N1-N9, was established. HA and NA genes were selected based on sequence analyses of influenza virus genes available from public databases. Prototype Eurasian HA and NA genes were cloned in bidirectional reverse genetics plasmids. Recombinant viruses based on the virus backbone of A/PR/8/34, and containing a variety of HA and NA genes were produced in 293T cells. Virus stocks were produced in MDCK cells and embryonated chicken eggs. These plasmids and viruses may be useful for numerous purposes, including influenza virus research projects, vaccination studies, and to serve as reference reagents in diagnostic settings. (C) 2010 Published by Elsevier Ltd.
AB - Reverse genetics can be used to produce recombinant influenza A viruses containing virtually every desired combination of hemagglutinin (HA) and neuraminidase (NA) genes using the virus backbone of choice. Here, a repository of plasmids and recombinant viruses representing all contemporary Eurasian HA and NA subtypes, H1-H16 and N1-N9, was established. HA and NA genes were selected based on sequence analyses of influenza virus genes available from public databases. Prototype Eurasian HA and NA genes were cloned in bidirectional reverse genetics plasmids. Recombinant viruses based on the virus backbone of A/PR/8/34, and containing a variety of HA and NA genes were produced in 293T cells. Virus stocks were produced in MDCK cells and embryonated chicken eggs. These plasmids and viruses may be useful for numerous purposes, including influenza virus research projects, vaccination studies, and to serve as reference reagents in diagnostic settings. (C) 2010 Published by Elsevier Ltd.
U2 - 10.1016/j.vaccine.2010.06.072
DO - 10.1016/j.vaccine.2010.06.072
M3 - Article
C2 - 20600474
SN - 0264-410X
VL - 28
SP - 5803
EP - 5809
JO - Vaccine
JF - Vaccine
IS - 36
ER -