Rescue of a H3N2 influenza virus containing a deficient neuraminidase protein by a hemagglutinin with a low receptor-binding affinity

Mathilde Richard*, Alexandra Erny, Bertrand Caré, Aurélien Traversier, Mendy Barthélémy, Alan Hay, Yi Pu Lin, Olivier Ferraris, Bruno Lina

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)
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Abstract

Influenza viruses possess at their surface two glycoproteins, the hemagglutinin and the neuraminidase, of which the antagonistic functions have to be well balanced for the virus to grow efficiently. Ferraris etal. isolated in 2003-2004 viruses lacking both a NA gene and protein (H3NA- viruses) (Ferraris O., 2006, Vaccine, 24(44-46):6656-9). In this study we showed that the hemagglutinins of two of the H3NA- viruses have reduced affinity for SAα2.6Gal receptors, between 49 and 128 times lower than that of the A/Moscow/10/99 (H3N2) virus and no detectable affinity for SAα2.3Gal receptors. We also showed that the low hemagglutinin affinity of the H3NA- viruses compensates for the lack of NA activity and allows the restoration of the growth of an A/Moscow/10/99 virus deficient in neuraminidase. These observations increase our understanding of H3NA- viruses in relation to the balance between the functional activities of the neuraminidase and hemagglutinin.

Original languageEnglish
Article numbere33880
JournalPLoS ONE
Volume7
Issue number5
DOIs
Publication statusPublished - 1 May 2012
Externally publishedYes

Bibliographical note

Funding:
This work was supported by the Centre National de Recherche Scientifique, the Groupement d’Etudes Immunologiques. Dr. Richard received a grant
from the Ministe`re de la Recherche. These funding sources had no role in study design, data collection, analysis and interpretation of the results, decision to
publish, and preparation of the manuscript.

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