Restricted Varicella-Zoster Virus Transcription in Human Trigeminal Ganglia Obtained Soon after Death

Werner Ouwendijk; A Choe; MA Nagel; D Gilden; Ab Osterhaus; RJ Cohrs; Georges Verjans

doi:10.1128/JVI.01331-12

Restricted Varicella-Zoster Virus Transcription in Human Trigeminal Ganglia Obtained Soon after Death

Werner Ouwendijk
, A Choe
, MA Nagel
, D Gilden
, Ab Osterhaus
, RJ Cohrs
, Georges Verjans

Virology

Research output: Contribution to journal › Article › Academic › peer-review

55 Citations (Scopus)

Abstract

We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.

Original language	Undefined/Unknown
Pages (from-to)	10203-10206
Number of pages	4
Journal	Journal of Virology
Volume	86
Issue number	18
DOIs	https://doi.org/10.1128/JVI.01331-12
Publication status	Published - 2012

Research programs

EMC MM-04-27-01

Access to Document

10.1128/JVI.01331-12

http://hdl.handle.net/1765/39046

Cite this

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title = "Restricted Varicella-Zoster Virus Transcription in Human Trigeminal Ganglia Obtained Soon after Death",

abstract = "We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.",

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AU - Ouwendijk, Werner

AU - Choe, A

AU - Nagel, MA

AU - Gilden, D

AU - Osterhaus, Ab

AU - Cohrs, RJ

AU - Verjans, Georges

PY - 2012

Y1 - 2012

N2 - We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.

AB - We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.

U2 - 10.1128/JVI.01331-12

DO - 10.1128/JVI.01331-12

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VL - 86

SP - 10203

EP - 10206

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JF - Journal of Virology

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