Retrotransposon insertion-site context (RISC) typing: a novel typing method for Aspergillus fumigatus and a convenient PCR alternative to restriction fragment length polymorphism analysis

Maaike T de Ruiter, Hanneke A de Valk, Jacques F G M Meis, Corné H W Klaassen

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Retrotransposon(-like) sequences in Aspergillus fumigatus have been used as typing targets through restriction fragment length polymorphism (RFLP)/Southern blotting approaches. Differences in fingerprints between unrelated isolates are the result of variations in copy-number and differences in the regions flanking the retrotransposon elements. Here, we present retrotransposon insertion-site context (RISC) typing as a novel and convenient PCR-based typing alternative to the RFLP approach. RISC typing aims at amplifying the sequences flanking the retrotransposon-like sequences in A. fumigatus and allows large numbers of isolates to be analyzed in a timely fashion with excellent discriminatory power.

Original languageEnglish
Pages (from-to)528-34
Number of pages7
JournalJournal of Microbiological Methods
Volume70
Issue number3
DOIs
Publication statusPublished - Sept 2007
Externally publishedYes

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