Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Anne Rahbech, Reno Debets, Per Thor Straten, Marlies J.W. Peeters

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.

Original languageEnglish
Pages (from-to)29-39
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume2748
DOIs
Publication statusPublished - 2024

Bibliographical note

Publisher Copyright:
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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