Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Anne Rahbech; Reno Debets; Per Thor Straten; Marlies J.W. Peeters

doi:10.1007/978-1-0716-3593-3_3

Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Anne Rahbech
, Reno Debets
, Per Thor Straten
, Marlies J.W. Peeters

Medical Oncology

Copenhagen University Hospital (Nordvest)
University of Copenhagen

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.

Original language	English
Pages (from-to)	29-39
Number of pages	11
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2748
DOIs	https://doi.org/10.1007/978-1-0716-3593-3_3
Publication status	Published - 2024

Bibliographical note

Publisher Copyright:
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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10.1007/978-1-0716-3593-3_3

Retroviral Transduction of Human Primary Final published version, 402 KBLicence: Article 25fa Dutch Copyright Act

Cite this

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abstract = "Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.",

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AU - Peeters, Marlies J.W.

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Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Abstract

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