Retroviral vectors for clinical immunogene therapy are stable for up to 9 years

Cor Lamers, Pascal Elzakker, BA Luider, SCL van Steenbergen, Stefan Sleijfer, Reno Debets, Jan willem Gratama

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Recombinant retroviruses are one of the most commonly used gene transfer vehicles for therapeutic gene delivery. The stability of viral vectors upon long-term storage, anticipated to be short lived, is expected to impact timeline and financial course of clinical immunogene therapy. However, to date little is known about vector stability. Therefore, we analyzed the stability of retroviral vectors produced in culture supernatants (RTVsup) for ex vivo gene therapy upon long-term storage. We have generated RTVsups derived from two packaging cell lines, PG13 and Phoenix(Ampho). Both lines produced murine leukemia virus-derived SFG-scFv(G250)-CD4 gamma vector, which were pseudotyped with the gibbon ape leukemia virus envelope and amphotropic envelope, respectively. The supernatants were stored at -80 or -196 degrees C. To date, the PG13-derived RTVsups have been evaluated over a period of 9 years ( 1998-2007). In addition, a clinical batch of Phoenix( Ampho)-derived RTVsup has been evaluated over a period of 5 years ( 2002-2007). Here, we show that both RTVsups, when stored up to 9 and 5 years, respectively, do not show any sign of decay in their capacity to functionally transduce primary human T cells. These data provide evidence that in terms of `life expectancy' the production and storage of clinical batches of RTVsup for gene therapy warrants the corresponding professional and financial risks.
Original languageUndefined/Unknown
Pages (from-to)268-274
Number of pages7
JournalCancer Gene Therapy
Volume15
Issue number4
DOIs
Publication statusPublished - 2008

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