Riboflavin and photoproducts in MC3T3-E1 differentiation

Antonio Hernandes Chaves Neto; Claudia Lumy Yano; Edgar Julian Paredes-Gamero; Daisy Machado; Giselle Zenker Justo; Maikel P. Peppelenbosch; Carmen Veríssima Ferreira

doi:10.1016/j.tiv.2010.07.026

Riboflavin and photoproducts in MC3T3-E1 differentiation

Antonio Hernandes Chaves Neto^*, Claudia Lumy Yano, Edgar Julian Paredes-Gamero, Daisy Machado, Giselle Zenker Justo, Maikel P. Peppelenbosch, Carmen Veríssima Ferreira

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

Photoderivatives of riboflavin can modulate the proliferation and survival of cancer cells. In this work, we examined the influence of riboflavin and photoderivatives on osteoblast differentiation induced by ascorbic acid and β-glycerophosphate. These compounds decreased the osteoblast proliferation, increased the alkaline phosphatase activity, promoted a reduction in matrix metalloproteinase-2 activity and the decreased in the OPG/RANKL ratio. The effects of flavins on osteoblasts were unrelated to the antioxidant activity of these compounds. The biological activity of osteogenic medium containing riboflavin and its photoderivatives involved the activation of different signaling pathways (AKT, FAK, CaMKII), caspases-3, -8 and -9, and up-regulation of the expression and/or stabilization of osteoblastic transcription factors (Runx2 and β-catenin). These findings suggest a potential use of flavins as adjuvants to improve bone metabolism.

Original language	English
Pages (from-to)	1911-1919
Number of pages	9
Journal	Toxicology in Vitro
Volume	24
Issue number	7
DOIs	https://doi.org/10.1016/j.tiv.2010.07.026
Publication status	Published - Oct 2010
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank Claudia L. Soraggi, Denise B. Ciampi, Erika Ferraresso dos Anjos and Rodrigo A. da Silva for technical support. A.H.C.N. was supported by a Ph.D. scholarship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Grant No. 06/00430-1 ).

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title = "Riboflavin and photoproducts in MC3T3-E1 differentiation",

abstract = "Photoderivatives of riboflavin can modulate the proliferation and survival of cancer cells. In this work, we examined the influence of riboflavin and photoderivatives on osteoblast differentiation induced by ascorbic acid and β-glycerophosphate. These compounds decreased the osteoblast proliferation, increased the alkaline phosphatase activity, promoted a reduction in matrix metalloproteinase-2 activity and the decreased in the OPG/RANKL ratio. The effects of flavins on osteoblasts were unrelated to the antioxidant activity of these compounds. The biological activity of osteogenic medium containing riboflavin and its photoderivatives involved the activation of different signaling pathways (AKT, FAK, CaMKII), caspases-3, -8 and -9, and up-regulation of the expression and/or stabilization of osteoblastic transcription factors (Runx2 and β-catenin). These findings suggest a potential use of flavins as adjuvants to improve bone metabolism.",

author = "\{Chaves Neto\}, \{Antonio Hernandes\} and Yano, \{Claudia Lumy\} and Paredes-Gamero, \{Edgar Julian\} and Daisy Machado and Justo, \{Giselle Zenker\} and Peppelenbosch, \{Maikel P.\} and Ferreira, \{Carmen Ver{\'i}ssima\}",

note = "Funding Information: The authors thank Claudia L. Soraggi, Denise B. Ciampi, Erika Ferraresso dos Anjos and Rodrigo A. da Silva for technical support. A.H.C.N. was supported by a Ph.D. scholarship from Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo (FAPESP, Grant No. 06/00430-1 ).",

year = "2010",

month = oct,

doi = "10.1016/j.tiv.2010.07.026",

language = "English",

volume = "24",

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journal = "Toxicology in Vitro",

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AU - Chaves Neto, Antonio Hernandes

AU - Yano, Claudia Lumy

AU - Paredes-Gamero, Edgar Julian

AU - Machado, Daisy

AU - Justo, Giselle Zenker

AU - Peppelenbosch, Maikel P.

AU - Ferreira, Carmen Veríssima

N1 - Funding Information: The authors thank Claudia L. Soraggi, Denise B. Ciampi, Erika Ferraresso dos Anjos and Rodrigo A. da Silva for technical support. A.H.C.N. was supported by a Ph.D. scholarship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Grant No. 06/00430-1 ).

PY - 2010/10

Y1 - 2010/10

N2 - Photoderivatives of riboflavin can modulate the proliferation and survival of cancer cells. In this work, we examined the influence of riboflavin and photoderivatives on osteoblast differentiation induced by ascorbic acid and β-glycerophosphate. These compounds decreased the osteoblast proliferation, increased the alkaline phosphatase activity, promoted a reduction in matrix metalloproteinase-2 activity and the decreased in the OPG/RANKL ratio. The effects of flavins on osteoblasts were unrelated to the antioxidant activity of these compounds. The biological activity of osteogenic medium containing riboflavin and its photoderivatives involved the activation of different signaling pathways (AKT, FAK, CaMKII), caspases-3, -8 and -9, and up-regulation of the expression and/or stabilization of osteoblastic transcription factors (Runx2 and β-catenin). These findings suggest a potential use of flavins as adjuvants to improve bone metabolism.

AB - Photoderivatives of riboflavin can modulate the proliferation and survival of cancer cells. In this work, we examined the influence of riboflavin and photoderivatives on osteoblast differentiation induced by ascorbic acid and β-glycerophosphate. These compounds decreased the osteoblast proliferation, increased the alkaline phosphatase activity, promoted a reduction in matrix metalloproteinase-2 activity and the decreased in the OPG/RANKL ratio. The effects of flavins on osteoblasts were unrelated to the antioxidant activity of these compounds. The biological activity of osteogenic medium containing riboflavin and its photoderivatives involved the activation of different signaling pathways (AKT, FAK, CaMKII), caspases-3, -8 and -9, and up-regulation of the expression and/or stabilization of osteoblastic transcription factors (Runx2 and β-catenin). These findings suggest a potential use of flavins as adjuvants to improve bone metabolism.

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JF - Toxicology in Vitro

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ER -

Riboflavin and photoproducts in MC3T3-E1 differentiation

Abstract

Bibliographical note

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