Role of Delta-like-4/Notch in the Formation and Wiring of the Lymphatic Network in Zebrafish

I Geudens; Robert Herpers; K Hermans; I Segura; CR de Almodovar; Hans Bussmann; F De Smet; W Vandevelde; BM Hogan; A Siekmann; F Claes; JC Moore; AS Pistocchi; S Loges; M Mazzone; G Mariggi; F Bruyere; F Cotelli; D Kerjaschki; A Noel; JM Foidart; H Gerhardt; A Ny; T Langenberg; ND Lawson; Eric Duckers; S Schulte-Merker; P Carmeliet; M Dewerchin

doi:10.1161/ATVBAHA.110.203034

Role of Delta-like-4/Notch in the Formation and Wiring of the Lymphatic Network in Zebrafish

I Geudens, Robert Herpers, K Hermans, I Segura, CR de Almodovar, Hans Bussmann, F De Smet, W Vandevelde, BM Hogan, A Siekmann, F Claes, JC Moore, AS Pistocchi, S Loges, M Mazzone, G Mariggi, F Bruyere, F Cotelli, D Kerjaschki, A NoelJM Foidart, H Gerhardt, A Ny, T Langenberg, ND Lawson, Eric Duckers, S Schulte-Merker, P Carmeliet, M Dewerchin

Research output: Contribution to journal › Article › Academic › peer-review

97 Citations (Scopus)

Abstract

Objective-To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. Methods and Results-In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. Conclusion-These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. (Arterioscler Thromb Vasc Biol. 2010; 30: 1695-1702.)

Original language	Undefined/Unknown
Pages (from-to)	1695-U60
Journal	Arteriosclerosis Thrombosis & Vascular Biology
Volume	30
Issue number	9
DOIs	https://doi.org/10.1161/ATVBAHA.110.203034
Publication status	Published - 2010

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EMC COEUR-09

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10.1161/ATVBAHA.110.203034

http://hdl.handle.net/1765/20853

Cite this

Geudens, I., Herpers, R., Hermans, K., Segura, I., de Almodovar, CR., Bussmann, H., De Smet, F., Vandevelde, W., Hogan, BM., Siekmann, A., Claes, F., Moore, JC., Pistocchi, AS., Loges, S., Mazzone, M., Mariggi, G., Bruyere, F., Cotelli, F., Kerjaschki, D., ... Dewerchin, M. (2010). Role of Delta-like-4/Notch in the Formation and Wiring of the Lymphatic Network in Zebrafish. Arteriosclerosis Thrombosis & Vascular Biology, 30(9), 1695-U60. https://doi.org/10.1161/ATVBAHA.110.203034

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title = "Role of Delta-like-4/Notch in the Formation and Wiring of the Lymphatic Network in Zebrafish",

abstract = "Objective-To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. Methods and Results-In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. Conclusion-These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. (Arterioscler Thromb Vasc Biol. 2010; 30: 1695-1702.)",

author = "I Geudens and Robert Herpers and K Hermans and I Segura and {de Almodovar}, CR and Hans Bussmann and {De Smet}, F and W Vandevelde and BM Hogan and A Siekmann and F Claes and JC Moore and AS Pistocchi and S Loges and M Mazzone and G Mariggi and F Bruyere and F Cotelli and D Kerjaschki and A Noel and JM Foidart and H Gerhardt and A Ny and T Langenberg and ND Lawson and Eric Duckers and S Schulte-Merker and P Carmeliet and M Dewerchin",

year = "2010",

doi = "10.1161/ATVBAHA.110.203034",

language = "Undefined/Unknown",

volume = "30",

pages = "1695--U60",

journal = "Arteriosclerosis Thrombosis & Vascular Biology",

issn = "1079-5642",

publisher = "Lippincott Williams & Wilkins",

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Geudens, I, Herpers, R, Hermans, K, Segura, I, de Almodovar, CR, Bussmann, H, De Smet, F, Vandevelde, W, Hogan, BM, Siekmann, A, Claes, F, Moore, JC, Pistocchi, AS, Loges, S, Mazzone, M, Mariggi, G, Bruyere, F, Cotelli, F, Kerjaschki, D, Noel, A, Foidart, JM, Gerhardt, H, Ny, A, Langenberg, T, Lawson, ND, Duckers, E, Schulte-Merker, S, Carmeliet, P & Dewerchin, M 2010, 'Role of Delta-like-4/Notch in the Formation and Wiring of the Lymphatic Network in Zebrafish', Arteriosclerosis Thrombosis & Vascular Biology, vol. 30, no. 9, pp. 1695-U60. https://doi.org/10.1161/ATVBAHA.110.203034

TY - JOUR

T1 - Role of Delta-like-4/Notch in the Formation and Wiring of the Lymphatic Network in Zebrafish

AU - Geudens, I

AU - Herpers, Robert

AU - Hermans, K

AU - Segura, I

AU - de Almodovar, CR

AU - Bussmann, Hans

AU - De Smet, F

AU - Vandevelde, W

AU - Hogan, BM

AU - Siekmann, A

AU - Claes, F

AU - Moore, JC

AU - Pistocchi, AS

AU - Loges, S

AU - Mazzone, M

AU - Mariggi, G

AU - Bruyere, F

AU - Cotelli, F

AU - Kerjaschki, D

AU - Noel, A

AU - Foidart, JM

AU - Gerhardt, H

AU - Ny, A

AU - Langenberg, T

AU - Lawson, ND

AU - Duckers, Eric

AU - Schulte-Merker, S

AU - Carmeliet, P

AU - Dewerchin, M

PY - 2010

Y1 - 2010

N2 - Objective-To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. Methods and Results-In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. Conclusion-These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. (Arterioscler Thromb Vasc Biol. 2010; 30: 1695-1702.)

AB - Objective-To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. Methods and Results-In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. Conclusion-These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. (Arterioscler Thromb Vasc Biol. 2010; 30: 1695-1702.)

U2 - 10.1161/ATVBAHA.110.203034

DO - 10.1161/ATVBAHA.110.203034

M3 - Article

SN - 1079-5642

VL - 30

SP - 1695-U60

JO - Arteriosclerosis Thrombosis & Vascular Biology

JF - Arteriosclerosis Thrombosis & Vascular Biology

IS - 9

ER -