Abstract
We have used high-resolution 4D imaging of cardiac progenitor cells (CPCs) in zebrafish to investigate the earliest left-right asymmetric movements during cardiac morphogenesis. Differential migratory behavior within the heart field was observed, resulting in a rotation of the heart tube. The leftward displacement and rotation of the tube requires hyaluronan synthase 2 expression within the CPCs. Furthermore, by reducing or ectopically activating BMP signaling or by implantation of BMP beads we could demonstrate that BMP signaling, which is asymmetrically activated in the lateral plate mesoderm and regulated by early left-right signals, is required to direct CPC migration and cardiac rotation. Together, these results support a model in which CPCs migrate toward a BMP source during development of the linear heart tube, providing a mechanism by which the left-right axis drives asymmetric development of the vertebrate heart.
| Original language | English |
|---|---|
| Pages (from-to) | 287-297 |
| Number of pages | 11 |
| Journal | Developmental Cell |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 12 Feb 2008 |
| Externally published | Yes |
Bibliographical note
Funding Information:We are grateful to D. Stainier and H.-J. Tsai for providing the tg(cmlc2:gfp) fish, J. Kuipers and M. Camacho for technical assistance, D. Yelon, J. Yost, and S. Abdelilah-Seyfried for sending plasmids, and A. Moorman and B. Hogan for critical reading of the manuscript and stimulating discussions. Work in J.B.'s laboratory was supported by the KNAW, by EU FP6 grant LSHM-CT-2005-018833, by NWO (ALW Grant #814.02.007), and by HFSP (Research Grant RGP9/2003). K.S. was supported by ICIN grant #39.740. J. Bussmann has been supported by a Boehringer Ingelheim Fonds PhD Scholarship.