Self-organization of myosin II in reconstituted actomyosin bundles

Matthew R. Stachowiak, Patrick M. McCall, Todd Thoresen, Hayri E. Balcioglu, Lisa Kasiewicz, Margaret L. Gardel, Ben O'Shaughnessy*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

37 Citations (Scopus)

Abstract

Cells assemble a variety of bundled actomyosin structures in the cytoskeleton for activities such as cell-shape regulation, force production, and cytokinesis. Although these linear structures exhibit varied architecture, two common organizational themes are a punctate distribution of myosin II and distinct patterns of actin polarity. The mechanisms that cells use to assemble and maintain these organizational features are poorly understood. To study these, we reconstituted actomyosin bundles in vitro that contained only actin filaments and myosin II. Upon addition of ATP, the bundles contracted and the uniformly distributed myosin spontaneously reorganized into discrete clusters. We developed a mathematical model in which the motion of myosin II filaments is governed by the polarities of the actin filaments with which they interact. The model showed that the assembly of myosins into clusters is driven by their tendency to migrate to locations with zero net actin filament polarity. With no fitting parameters, the predicted distribution of myosin cluster separations was in close agreement with our experiments, including a -3/2 power law decay for intermediate length scales. Thus, without an organizing template or accessory proteins, a minimal bundle of actin and myosin has the inherent capacity to self-organize into a heterogeneous banded structure.

Original languageEnglish
Pages (from-to)1265-1274
Number of pages10
JournalBiophysical Journal
Volume103
Issue number6
DOIs
Publication statusPublished - 19 Sept 2012
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health grants GM086731 (B.O.) and DP10D00354 (M.L.G.).

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