Seroepidemiology of Breda virus in cattle using ELISA

M. Koopmans; U. Van Den Boom; G. Woode; M. C. Horzinek

doi:10.1016/0378-1135(89)90069-2

Seroepidemiology of Breda virus in cattle using ELISA

M. Koopmans^*, U. Van Den Boom, G. Woode, M. C. Horzinek

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

34 Citations (Scopus)

Abstract

Two direct blocking enzyme linked immunosorbent assays (ELISA) for the detection of antibodies to Breda virus in sera of cattle were compared. An ELISA with consecutive addition of antigen and test serum to an antibody-coated plate gave higher positive: negative absorbence ratios than an ELISA in which antigen and test serum were added simultaneously. Sera collected from breeding and fattening herds in The Netherlands (n = 1313) and the F.R.G. (n = 716) were tested, and antibodies to Breda virus were demonstrated in 94% of adult cattle. Ninety percent of newborn calves had high levels of maternal antibodies, which waned until the age of 3 months. Active seroconversion occurred between 7 and 24 months in most animals.

Original language	English
Pages (from-to)	233-243
Number of pages	11
Journal	Veterinary Microbiology
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/0378-1135(89)90069-2
Publication status	Published - Mar 1989
Externally published	Yes

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title = "Seroepidemiology of Breda virus in cattle using ELISA",

abstract = "Two direct blocking enzyme linked immunosorbent assays (ELISA) for the detection of antibodies to Breda virus in sera of cattle were compared. An ELISA with consecutive addition of antigen and test serum to an antibody-coated plate gave higher positive: negative absorbence ratios than an ELISA in which antigen and test serum were added simultaneously. Sera collected from breeding and fattening herds in The Netherlands (n = 1313) and the F.R.G. (n = 716) were tested, and antibodies to Breda virus were demonstrated in 94% of adult cattle. Ninety percent of newborn calves had high levels of maternal antibodies, which waned until the age of 3 months. Active seroconversion occurred between 7 and 24 months in most animals.",

author = "M. Koopmans and {Van Den Boom}, U. and G. Woode and Horzinek, {M. C.}",

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AU - Van Den Boom, U.

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AU - Horzinek, M. C.

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AB - Two direct blocking enzyme linked immunosorbent assays (ELISA) for the detection of antibodies to Breda virus in sera of cattle were compared. An ELISA with consecutive addition of antigen and test serum to an antibody-coated plate gave higher positive: negative absorbence ratios than an ELISA in which antigen and test serum were added simultaneously. Sera collected from breeding and fattening herds in The Netherlands (n = 1313) and the F.R.G. (n = 716) were tested, and antibodies to Breda virus were demonstrated in 94% of adult cattle. Ninety percent of newborn calves had high levels of maternal antibodies, which waned until the age of 3 months. Active seroconversion occurred between 7 and 24 months in most animals.

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Seroepidemiology of Breda virus in cattle using ELISA

Abstract

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