Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

Willem van Wamel; Silvie Hansenová Maňásková; AC Fluit; Henri Verbrugh; AJ de Neeling; E van Duijkeren; Alex Belkum

doi:10.1007/s10096-009-0816-3

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

Willem van Wamel, Silvie Hansenová Maňásková, AC Fluit, Henri Verbrugh, AJ de Neeling, E van Duijkeren, Alex Belkum

Medical Microbiology & Infectious Diseases

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Abstract

Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

Original language	English
Pages (from-to)	119-122
Number of pages	4
Journal	European Journal of Clinical Microbiology & Infectious Diseases
Volume	29
Issue number	1
Early online date	2 Oct 2009
DOIs	https://doi.org/10.1007/s10096-009-0816-3
Publication status	Published - 2010

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EMC MM-04-28-01

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http://hdl.handle.net/1765/17240

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van Wamel, W., Hansenová Maňásková, S., Fluit, AC., Verbrugh, H., de Neeling, AJ., van Duijkeren, E., & Belkum, A. (2010). Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398. European Journal of Clinical Microbiology & Infectious Diseases, 29(1), 119-122. https://doi.org/10.1007/s10096-009-0816-3

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abstract = "Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.",

author = "{van Wamel}, Willem and {Hansenov{\'a} Ma{\v n}{\'a}skov{\'a}}, Silvie and AC Fluit and Henri Verbrugh and {de Neeling}, AJ and {van Duijkeren}, E and Alex Belkum",

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van Wamel, W , Hansenová Maňásková, S, Fluit, AC, Verbrugh, H, de Neeling, AJ, van Duijkeren, E & Belkum, A 2010, 'Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398', European Journal of Clinical Microbiology & Infectious Diseases, vol. 29, no. 1, pp. 119-122. https://doi.org/10.1007/s10096-009-0816-3

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398. / van Wamel, Willem ; Hansenová Maňásková, Silvie; Fluit, AC et al.
In: European Journal of Clinical Microbiology & Infectious Diseases, Vol. 29, No. 1, 2010, p. 119-122.

Research output: Contribution to journal › Article › Academic › peer-review

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T1 - Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

AU - van Wamel, Willem

AU - Hansenová Maňásková, Silvie

AU - Fluit, AC

AU - Verbrugh, Henri

AU - de Neeling, AJ

AU - van Duijkeren, E

AU - Belkum, Alex

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AB - Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

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DO - 10.1007/s10096-009-0816-3

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C2 - 19795142

SN - 0934-9723

VL - 29

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JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

IS - 1

ER -

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

Abstract

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