Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

Willem van Wamel; Silvie Hansenová Maňásková; AC Fluit; Henri Verbrugh; AJ de Neeling; E van Duijkeren; Alex Belkum

doi:10.1007/s10096-009-0816-3

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

Willem van Wamel
, Silvie Hansenová Maňásková
, AC Fluit
, Henri Verbrugh
, AJ de Neeling
, E van Duijkeren
, Alex Belkum

Medical Microbiology & Infectious Diseases

Research output: Contribution to journal › Article › Academic › peer-review

53 Citations (Scopus)

65 Downloads (Pure)

Abstract

Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

Original language	English
Pages (from-to)	119-122
Number of pages	4
Journal	European Journal of Clinical Microbiology & Infectious Diseases
Volume	29
Issue number	1
Early online date	2 Oct 2009
DOIs	https://doi.org/10.1007/s10096-009-0816-3
Publication status	Published - 2010

Research programs

EMC MM-04-28-01

Access to Document

10.1007/s10096-009-0816-3Licence: CC BY-NC

OA_19795142.pdfFinal published version, 107 KBLicence: CC BY-NC
ShortTermMicroEvolution_2010.pdfFinal published version, 107 KBLicence: CC BY-NC

http://hdl.handle.net/1765/17240

Cite this

van Wamel, W., Hansenová Maňásková, S., Fluit, AC., Verbrugh, H., de Neeling, AJ., van Duijkeren, E., & Belkum, A. (2010). Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398. European Journal of Clinical Microbiology & Infectious Diseases, 29(1), 119-122. https://doi.org/10.1007/s10096-009-0816-3

@article{6e6632f3c810419b98057439752eaf99,

title = "Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398",

abstract = "Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100\% specificity and high sensitivity.",

author = "\{van Wamel\}, Willem and \{Hansenov{\'a} Ma{\v n}{\'a}skov{\'a}\}, Silvie and AC Fluit and Henri Verbrugh and \{de Neeling\}, AJ and \{van Duijkeren\}, E and Alex Belkum",

year = "2010",

doi = "10.1007/s10096-009-0816-3",

language = "English",

volume = "29",

pages = "119--122",

journal = "European Journal of Clinical Microbiology \& Infectious Diseases",

issn = "0934-9723",

publisher = "Springer Science+Business Media",

number = "1",

}

van Wamel, W , Hansenová Maňásková, S, Fluit, AC, Verbrugh, H, de Neeling, AJ, van Duijkeren, E & Belkum, A 2010, 'Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398', European Journal of Clinical Microbiology & Infectious Diseases, vol. 29, no. 1, pp. 119-122. https://doi.org/10.1007/s10096-009-0816-3

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398. / van Wamel, Willem ; Hansenová Maňásková, Silvie; Fluit, AC et al.
In: European Journal of Clinical Microbiology & Infectious Diseases, Vol. 29, No. 1, 2010, p. 119-122.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

AU - van Wamel, Willem

AU - Hansenová Maňásková, Silvie

AU - Fluit, AC

AU - Verbrugh, Henri

AU - de Neeling, AJ

AU - van Duijkeren, E

AU - Belkum, Alex

PY - 2010

Y1 - 2010

N2 - Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

AB - Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

U2 - 10.1007/s10096-009-0816-3

DO - 10.1007/s10096-009-0816-3

M3 - Article

C2 - 19795142

SN - 0934-9723

VL - 29

SP - 119

EP - 122

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

IS - 1

ER -

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

Abstract

Research programs

Access to Document

Fingerprint

Cite this