Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

Ursula S.A. Stalmann; Bella Banjanin; Inge A.M. Snoeren; James S. Nagai; Nils B. Leimkühler; Ronghui Li; Adam Benabid; Jessica E. Pritchard; Hanna Malyaran; Sabine Neuss; Eric M. Bindels; Ivan G. Costa; Rebekka K. Schneider

doi:10.1016/j.exphem.2022.03.010

Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

Ursula S.A. Stalmann, Bella Banjanin, Inge A.M. Snoeren, James S. Nagai, Nils B. Leimkühler, Ronghui Li, Adam Benabid, Jessica E. Pritchard, Hanna Malyaran, Sabine Neuss, Eric M. Bindels, Ivan G. Costa, Rebekka K. Schneider^*

^*Corresponding author for this work

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Abstract

Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.

Original language	English
Pages (from-to)	28-33
Number of pages	6
Journal	Experimental Hematology
Volume	110
Early online date	24 Mar 2022
DOIs	https://doi.org/10.1016/j.exphem.2022.03.010
Publication status	Published - 1 Jun 2022

Bibliographical note

Funding Information:
RKS is an Oncode Institute investigator and was supported by grants from the MPN Foundation (2017 MPNRF/LLS Award), a KWF Kankerbestrijding Young Investigator Grant (11031/2017–1, Bas Mulder Award, Dutch Cancer Foundation), and an ERC grant (deFIBER; ERC-StG 757339). This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) to RKS (SCHN1188/6–1) and IGC (GE2811/4-1) within the clinical research unit CRU344. IGC and RKS are members of the E:MED Consortia Fibromap funded by the German Ministry of Education and Science (BMBF). US was supported by a research fellowship by the IMF University of Münster (ST521701) and by a research fellowship from the DFG (STA 1648/1–1).

Publisher Copyright:
© 2022 ISEH – Society for Hematology and Stem Cells

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ingle-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situFinal published version, 1.78 MBLicence: CC BY

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Stalmann, U. S. A., Banjanin, B., Snoeren, I. A. M., Nagai, J. S., Leimkühler, N. B., Li, R., Benabid, A., Pritchard, J. E., Malyaran, H., Neuss, S., Bindels, E. M., Costa, I. G., & Schneider, R. K. (2022). Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ. Experimental Hematology, 110, 28-33. https://doi.org/10.1016/j.exphem.2022.03.010

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title = "Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ",

abstract = "Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.",

author = "Stalmann, {Ursula S.A.} and Bella Banjanin and Snoeren, {Inge A.M.} and Nagai, {James S.} and Leimk{\"u}hler, {Nils B.} and Ronghui Li and Adam Benabid and Pritchard, {Jessica E.} and Hanna Malyaran and Sabine Neuss and Bindels, {Eric M.} and Costa, {Ivan G.} and Schneider, {Rebekka K.}",

note = "Funding Information: RKS is an Oncode Institute investigator and was supported by grants from the MPN Foundation (2017 MPNRF/LLS Award), a KWF Kankerbestrijding Young Investigator Grant (11031/2017–1, Bas Mulder Award, Dutch Cancer Foundation), and an ERC grant (deFIBER; ERC-StG 757339). This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) to RKS (SCHN1188/6–1) and IGC (GE2811/4-1) within the clinical research unit CRU344. IGC and RKS are members of the E:MED Consortia Fibromap funded by the German Ministry of Education and Science (BMBF). US was supported by a research fellowship by the IMF University of M{\"u}nster (ST521701) and by a research fellowship from the DFG (STA 1648/1–1). Publisher Copyright: {\textcopyright} 2022 ISEH – Society for Hematology and Stem Cells",

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Stalmann, USA, Banjanin, B, Snoeren, IAM, Nagai, JS, Leimkühler, NB, Li, R, Benabid, A, Pritchard, JE, Malyaran, H, Neuss, S, Bindels, EM, Costa, IG & Schneider, RK 2022, 'Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ', Experimental Hematology, vol. 110, pp. 28-33. https://doi.org/10.1016/j.exphem.2022.03.010

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T1 - Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

AU - Stalmann, Ursula S.A.

AU - Banjanin, Bella

AU - Snoeren, Inge A.M.

AU - Nagai, James S.

AU - Leimkühler, Nils B.

AU - Li, Ronghui

AU - Benabid, Adam

AU - Pritchard, Jessica E.

AU - Malyaran, Hanna

AU - Neuss, Sabine

AU - Bindels, Eric M.

AU - Costa, Ivan G.

AU - Schneider, Rebekka K.

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Y1 - 2022/6/1

N2 - Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.

AB - Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.

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AN - SCOPUS:85128168509

SN - 0301-472X

VL - 110

SP - 28

EP - 33

JO - Experimental Hematology

JF - Experimental Hematology

ER -

Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

Abstract

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