Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.
|Number of pages||6|
|Early online date||24 Mar 2022|
|Publication status||Published - 1 Jun 2022|
Bibliographical noteFunding Information:
RKS is an Oncode Institute investigator and was supported by grants from the MPN Foundation (2017 MPNRF/LLS Award), a KWF Kankerbestrijding Young Investigator Grant (11031/2017–1, Bas Mulder Award, Dutch Cancer Foundation), and an ERC grant (deFIBER; ERC-StG 757339). This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) to RKS (SCHN1188/6–1) and IGC (GE2811/4-1) within the clinical research unit CRU344. IGC and RKS are members of the E:MED Consortia Fibromap funded by the German Ministry of Education and Science (BMBF). US was supported by a research fellowship by the IMF University of Münster (ST521701) and by a research fellowship from the DFG (STA 1648/1–1).
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