Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

Ursula S.A. Stalmann, Bella Banjanin, Inge A.M. Snoeren, James S. Nagai, Nils B. Leimkühler, Ronghui Li, Adam Benabid, Jessica E. Pritchard, Hanna Malyaran, Sabine Neuss, Eric M. Bindels, Ivan G. Costa, Rebekka K. Schneider*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)
36 Downloads (Pure)

Abstract

Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalExperimental Hematology
Volume110
Early online date24 Mar 2022
DOIs
Publication statusPublished - 1 Jun 2022

Bibliographical note

Funding Information:
RKS is an Oncode Institute investigator and was supported by grants from the MPN Foundation (2017 MPNRF/LLS Award), a KWF Kankerbestrijding Young Investigator Grant (11031/2017–1, Bas Mulder Award, Dutch Cancer Foundation), and an ERC grant (deFIBER; ERC-StG 757339). This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) to RKS (SCHN1188/6–1) and IGC (GE2811/4-1) within the clinical research unit CRU344. IGC and RKS are members of the E:MED Consortia Fibromap funded by the German Ministry of Education and Science (BMBF). US was supported by a research fellowship by the IMF University of Münster (ST521701) and by a research fellowship from the DFG (STA 1648/1–1).

Publisher Copyright:
© 2022 ISEH – Society for Hematology and Stem Cells

Fingerprint

Dive into the research topics of 'Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ'. Together they form a unique fingerprint.

Cite this