Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

Ursula S.A. Stalmann; Bella Banjanin; Inge A.M. Snoeren; James S. Nagai; Nils B. Leimkühler; Ronghui Li; Adam Benabid; Jessica E. Pritchard; Hanna Malyaran; Sabine Neuss; Eric M. Bindels; Ivan G. Costa; Rebekka K. Schneider

doi:10.1016/j.exphem.2022.03.010

Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

Ursula S.A. Stalmann
, Bella Banjanin
, Inge A.M. Snoeren
, James S. Nagai
, Nils B. Leimkühler
, Ronghui Li
, Adam Benabid
, Jessica E. Pritchard
, Hanna Malyaran
, Sabine Neuss
, Eric M. Bindels
, Ivan G. Costa
, Rebekka K. Schneider^*

^*Corresponding author for this work

RWTH Aachen University
University Hospital Essen
Oncode Institute (Nederland)

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.

Original language	English
Pages (from-to)	28-33
Number of pages	6
Journal	Experimental Hematology
Volume	110
Early online date	24 Mar 2022
DOIs	https://doi.org/10.1016/j.exphem.2022.03.010
Publication status	Published - 1 Jun 2022

Bibliographical note

Funding Information:
RKS is an Oncode Institute investigator and was supported by grants from the MPN Foundation (2017 MPNRF/LLS Award), a KWF Kankerbestrijding Young Investigator Grant (11031/2017–1, Bas Mulder Award, Dutch Cancer Foundation), and an ERC grant (deFIBER; ERC-StG 757339). This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) to RKS (SCHN1188/6–1) and IGC (GE2811/4-1) within the clinical research unit CRU344. IGC and RKS are members of the E:MED Consortia Fibromap funded by the German Ministry of Education and Science (BMBF). US was supported by a research fellowship by the IMF University of Münster (ST521701) and by a research fellowship from the DFG (STA 1648/1–1).

Publisher Copyright:
© 2022 ISEH – Society for Hematology and Stem Cells

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ingle-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situFinal published version, 1.78 MBLicence: CC BY

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Stalmann, U. S. A., Banjanin, B., Snoeren, I. A. M., Nagai, J. S., Leimkühler, N. B., Li, R., Benabid, A., Pritchard, J. E., Malyaran, H., Neuss, S., Bindels, E. M., Costa, I. G., & Schneider, R. K. (2022). Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ. Experimental Hematology, 110, 28-33. https://doi.org/10.1016/j.exphem.2022.03.010

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title = "Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ",

abstract = "Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis{\textendash}stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.",

author = "Stalmann, \{Ursula S.A.\} and Bella Banjanin and Snoeren, \{Inge A.M.\} and Nagai, \{James S.\} and Leimk{\"u}hler, \{Nils B.\} and Ronghui Li and Adam Benabid and Pritchard, \{Jessica E.\} and Hanna Malyaran and Sabine Neuss and Bindels, \{Eric M.\} and Costa, \{Ivan G.\} and Schneider, \{Rebekka K.\}",

note = "Funding Information: RKS is an Oncode Institute investigator and was supported by grants from the MPN Foundation (2017 MPNRF/LLS Award), a KWF Kankerbestrijding Young Investigator Grant (11031/2017{\textendash}1, Bas Mulder Award, Dutch Cancer Foundation), and an ERC grant (deFIBER; ERC-StG 757339). This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) to RKS (SCHN1188/6{\textendash}1) and IGC (GE2811/4-1) within the clinical research unit CRU344. IGC and RKS are members of the E:MED Consortia Fibromap funded by the German Ministry of Education and Science (BMBF). US was supported by a research fellowship by the IMF University of M{\"u}nster (ST521701) and by a research fellowship from the DFG (STA 1648/1{\textendash}1). Publisher Copyright: {\textcopyright} 2022 ISEH {\textendash} Society for Hematology and Stem Cells",

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Stalmann, USA, Banjanin, B, Snoeren, IAM, Nagai, JS, Leimkühler, NB, Li, R, Benabid, A, Pritchard, JE, Malyaran, H, Neuss, S, Bindels, EM, Costa, IG & Schneider, RK 2022, 'Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ', Experimental Hematology, vol. 110, pp. 28-33. https://doi.org/10.1016/j.exphem.2022.03.010

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T1 - Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

AU - Stalmann, Ursula S.A.

AU - Banjanin, Bella

AU - Snoeren, Inge A.M.

AU - Nagai, James S.

AU - Leimkühler, Nils B.

AU - Li, Ronghui

AU - Benabid, Adam

AU - Pritchard, Jessica E.

AU - Malyaran, Hanna

AU - Neuss, Sabine

AU - Bindels, Eric M.

AU - Costa, Ivan G.

AU - Schneider, Rebekka K.

N1 - Funding Information: RKS is an Oncode Institute investigator and was supported by grants from the MPN Foundation (2017 MPNRF/LLS Award), a KWF Kankerbestrijding Young Investigator Grant (11031/2017–1, Bas Mulder Award, Dutch Cancer Foundation), and an ERC grant (deFIBER; ERC-StG 757339). This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) to RKS (SCHN1188/6–1) and IGC (GE2811/4-1) within the clinical research unit CRU344. IGC and RKS are members of the E:MED Consortia Fibromap funded by the German Ministry of Education and Science (BMBF). US was supported by a research fellowship by the IMF University of Münster (ST521701) and by a research fellowship from the DFG (STA 1648/1–1). Publisher Copyright: © 2022 ISEH – Society for Hematology and Stem Cells

PY - 2022/6/1

Y1 - 2022/6/1

N2 - Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.

AB - Within the heterogenous pool of bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest because of their hematopoiesis-supporting capacities, contribution to disease progression, therapy resistance, and leukemic initiation. Cultured bone marrow-derived stromal cells (cBMSCs) are used for in vitro modeling of hematopoiesis–stroma interactions, validation of disease mechanisms, and screening for therapeutic targets. Here, we place cBMSCs (mouse and human) in a bone marrow tissue context by systematically comparing the transcriptome of plastic-adherent cells on a single-cell level with in vivo counterparts. Cultured BMSCs encompass a rather homogenous cell population, independent of the isolation method used and, although still possessing hematopoiesis-supporting capacity, are distinct from freshly isolated MSCs and more akin to in vivo fibroblast populations.

UR - https://www.scopus.com/pages/publications/85128168509

U2 - 10.1016/j.exphem.2022.03.010

DO - 10.1016/j.exphem.2022.03.010

M3 - Article

C2 - 35341805

AN - SCOPUS:85128168509

SN - 0301-472X

VL - 110

SP - 28

EP - 33

JO - Experimental Hematology

JF - Experimental Hematology

ER -

Single-cell analysis of cultured bone marrow stromal cells reveals high similarity to fibroblasts in situ

Abstract

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