Single-cell rna sequencing reveals heterogeneity and functional diversity of lymphatic endothelial cells

Hannah Den Braanker, Astrid C. van Stigt, Marc R. Kok, Erik Lubberts, Radjesh J. Bisoendial*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

11 Citations (Scopus)
138 Downloads (Pure)


Lymphatic endothelial cells (LECs) line the lymphatic vasculature and play a central role in the immune response. LECs have abilities to regulate immune transport, to promote immune cell survival, and to cross present antigens to dendritic cells. Single-cell RNA sequencing (scRNA) technology has accelerated new discoveries in the field of lymphatic vascular biology. This review will summarize these new findings in regard to embryonic development, LEC heterogeneity with associated functional diversity, and interactions with other cells. Depending on the organ, location in the lymphatic vascular tree, and micro-environmental conditions, LECs feature unique properties and tasks. Furthermore, adjacent stromal cells need the support of LECs for fulfilling their tasks in the immune response, such as immune cell transport and antigen presentation. Although aberrant lymphatic vasculature has been observed in a number of chronic inflammatory diseases, the knowledge on LEC heterogeneity and functional diversity in these diseases is limited. Combining scRNA sequencing data with imaging and more in-depth functional experiments will advance our knowledge of LECs in health and disease. Building the case, the LEC could be put forward as a new therapeutic target in chronic inflammatory diseases, counterweighting the current immune-cell focused therapies.

Original languageEnglish
Article number11976
JournalInternational Journal of Molecular Sciences
Issue number21
Publication statusPublished - 5 Nov 2021

Bibliographical note

Funding Information:
Funding: This research received no external funding. The APC was funded by the Maasstad Hospital, Rotterdam, The Netherlands.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.


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