Skin Microbiota Profiles from Tape Stripping and Skin Biopsy Samples of Patients with Psoriasis Treated with Narrowband Ultraviolet B

Atiya Rungjang, Jitlada Meephansan*, Sunchai Payungporn, Vorthon Sawaswong, Prangwalai Chanchaem, Purit Pureesrisak, Jongkonnee Wongpiyabovorn, Hok Bing Thio

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)
24 Downloads (Pure)

Abstract

Purpose: Although the pathogenesis of psoriasis involves the dermis, most previous studies collected samples using the swab technique. A recent study examining the microbiomes obtained via both skin biopsies and swabs revealed a significant difference in normal skin. We hypothesized that the microbiome profile of patients with psoriasis from tape stripping and skin biopsy might be different. This study sought to contribute to microbiome research on psoriasis by investigating the changes in the microbiome during narrowband ultraviolet B (NBUVB) therapy by comparing the results from the different sampling techniques of tape stripping and skin biopsy. Patients and Methods: Twenty-three participants, including 14 patients with chronic plaque psoriasis and nine healthy controls, were recruited, and nine patients with psoriasis completed 20-sessions of NBUVB treatment. Skin microbiota from both techniques was analyzed using the 16S rRNA gene at baseline and after treatment. Results: A clear difference was observed between the results from the two sampling techniques. Alpha diversity of the microbiota obtained from tape stripping was higher than that of the microbiota from skin biopsy, whereas beta diversity was clustered into two groups by sampling technique. The microbiome was altered during NBUVB treatment using both sampling techniques. Conclusion: Different sampling techniques resulted in different microbiome profiles in patients with psoriasis. Tape stripping and swabs are feasible procedures and are mostly used in psoriasis and other skin microbiome studies; however, skin biopsy may also expand our understanding of psoriasis and other skin diseases that pathophysiology involves deeper to the dermis or subcutaneous tissue.

Original languageEnglish
Pages (from-to)1767-1778
Number of pages12
JournalClinical, Cosmetic and Investigational Dermatology
Volume15
DOIs
Publication statusPublished - 30 Aug 2022

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support provided by Chulabhorn International College of Medicine contract no T1/2563 and Bualuang ASEAN Chair Professorship Fund. We would like to thank Editage (www.editage.

Publisher Copyright:
© 2022 Rungjang et al.

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