Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa: Study Design and Methodology of the SAMPAN Study

Anneloes van Veen; Selvi N. Shahab; Amber Rijfkogel; Anne F. Voor in ’t holt; Corné H.W. Klaassen; Margreet C. Vos; Yulia Rosa Saharman; Anis Karuniawati; Silvia Zelli; Desy De Lorenzis; Giulia Menchinelli; Giulia De Angelis; Maurizio Sanguinetti; Merel Kemper; Anniek E.E. de Jong; Sima Mohammadi; Valentine Renaud; Irena Kukavica-Ibrulj; Marianne Potvin; Guillaume Q. Nguyen; Jeff Gauthier; Roger C. Levesque; Heike Schmitt; Juliëtte A. Severin

doi:10.3390/antibiotics14010094

Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa: Study Design and Methodology of the SAMPAN Study

Anneloes van Veen, Selvi N. Shahab, Amber Rijfkogel, Anne F. Voor in ’t holt, Corné H.W. Klaassen, Margreet C. Vos, Yulia Rosa Saharman, Anis Karuniawati, Silvia Zelli, Desy De Lorenzis, Giulia Menchinelli, Giulia De Angelis, Maurizio Sanguinetti, Merel Kemper, Anniek E.E. de Jong, Sima Mohammadi, Valentine Renaud, Irena Kukavica-Ibrulj, Marianne Potvin, Guillaume Q. NguyenJeff Gauthier, Roger C. Levesque, Heike Schmitt, Juliëtte A. Severin^*

^*Corresponding author for this work

Medical Microbiology & Infectious Diseases

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Abstract

Background/Objectives: The global spread of carbapenem-resistant Pseudomonas aeruginosa (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insufficient knowledge on the sources and transmission routes of CRPA. The aim of the SAMPAN study (“A Smart Surveillance Strategy for Carbapenem-resistant Pseudomonas aeruginosa”) is to develop a globally applicable surveillance strategy. Methods: First, an international cross-sectional study will be conducted to investigate CRPA in clinical and environmental settings in Rotterdam (The Netherlands), Rome (Italy), and Jakarta (Indonesia). Screening cultures and risk factor questionnaires will be taken from healthy individuals and patients upon hospital admission. Clinical CRPA isolates will also be included. Additionally, samples will be taken twice from wet hospital environments and monthly from the hospitals’ (drinking) water system, hospital and municipal wastewater treatment plants, and receiving rivers. Whole-genome sequencing will be performed to characterize CRPA isolates and determine the genetic relatedness among the isolates from different reservoirs. Findings from the cross-sectional study, combined with expert elicitation using a Delphi method, will serve as the input for the surveillance strategy. Conclusions: The SAMPAN study will provide a broader understanding of the sources and transmission routes of CRPA. Therewith, the development of a globally applicable smart surveillance strategy will be made possible, delivering information that is needed to guide actions against the spread of CRPA.

Original language	English
Article number	94
Journal	Antibiotics
Volume	14
Issue number	1
DOIs	https://doi.org/10.3390/antibiotics14010094
Publication status	Published - 15 Jan 2025

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van Veen, A., Shahab, S. N., Rijfkogel, A., Voor in ’t holt, A. F., Klaassen, C. H. W., Vos, M. C., Saharman, Y. R., Karuniawati, A., Zelli, S., De Lorenzis, D., Menchinelli, G., De Angelis, G., Sanguinetti, M., Kemper, M., de Jong, A. E. E., Mohammadi, S., Renaud, V., Kukavica-Ibrulj, I., Potvin, M., ... Severin, J. A. (2025). Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa: Study Design and Methodology of the SAMPAN Study. Antibiotics, 14(1), Article 94. https://doi.org/10.3390/antibiotics14010094

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title = "Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa: Study Design and Methodology of the SAMPAN Study",

abstract = "Background/Objectives: The global spread of carbapenem-resistant Pseudomonas aeruginosa (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insufficient knowledge on the sources and transmission routes of CRPA. The aim of the SAMPAN study (“A Smart Surveillance Strategy for Carbapenem-resistant Pseudomonas aeruginosa”) is to develop a globally applicable surveillance strategy. Methods: First, an international cross-sectional study will be conducted to investigate CRPA in clinical and environmental settings in Rotterdam (The Netherlands), Rome (Italy), and Jakarta (Indonesia). Screening cultures and risk factor questionnaires will be taken from healthy individuals and patients upon hospital admission. Clinical CRPA isolates will also be included. Additionally, samples will be taken twice from wet hospital environments and monthly from the hospitals{\textquoteright} (drinking) water system, hospital and municipal wastewater treatment plants, and receiving rivers. Whole-genome sequencing will be performed to characterize CRPA isolates and determine the genetic relatedness among the isolates from different reservoirs. Findings from the cross-sectional study, combined with expert elicitation using a Delphi method, will serve as the input for the surveillance strategy. Conclusions: The SAMPAN study will provide a broader understanding of the sources and transmission routes of CRPA. Therewith, the development of a globally applicable smart surveillance strategy will be made possible, delivering information that is needed to guide actions against the spread of CRPA.",

author = "{van Veen}, Anneloes and Shahab, {Selvi N.} and Amber Rijfkogel and {Voor in {\textquoteright}t holt}, {Anne F.} and Klaassen, {Corn{\'e} H.W.} and Vos, {Margreet C.} and Saharman, {Yulia Rosa} and Anis Karuniawati and Silvia Zelli and {De Lorenzis}, Desy and Giulia Menchinelli and {De Angelis}, Giulia and Maurizio Sanguinetti and Merel Kemper and {de Jong}, {Anniek E.E.} and Sima Mohammadi and Valentine Renaud and Irena Kukavica-Ibrulj and Marianne Potvin and Nguyen, {Guillaume Q.} and Jeff Gauthier and Levesque, {Roger C.} and Heike Schmitt and Severin, {Juli{\"e}tte A.}",

note = "Publisher Copyright: {\textcopyright} 2025 by the authors.",

year = "2025",

month = jan,

day = "15",

doi = "10.3390/antibiotics14010094",

language = "English",

volume = "14",

journal = "Antibiotics",

issn = "2079-6382",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "1",

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van Veen, A , Shahab, SN, Rijfkogel, A, Voor in ’t holt, AF , Klaassen, CHW , Vos, MC, Saharman, YR, Karuniawati, A, Zelli, S, De Lorenzis, D, Menchinelli, G, De Angelis, G, Sanguinetti, M, Kemper, M, de Jong, AEE, Mohammadi, S, Renaud, V, Kukavica-Ibrulj, I, Potvin, M, Nguyen, GQ, Gauthier, J, Levesque, RC, Schmitt, H & Severin, JA 2025, 'Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa: Study Design and Methodology of the SAMPAN Study', Antibiotics, vol. 14, no. 1, 94. https://doi.org/10.3390/antibiotics14010094

TY - JOUR

T1 - Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa

T2 - Study Design and Methodology of the SAMPAN Study

AU - van Veen, Anneloes

AU - Shahab, Selvi N.

AU - Rijfkogel, Amber

AU - Voor in ’t holt, Anne F.

AU - Klaassen, Corné H.W.

AU - Vos, Margreet C.

AU - Saharman, Yulia Rosa

AU - Karuniawati, Anis

AU - Zelli, Silvia

AU - De Lorenzis, Desy

AU - Menchinelli, Giulia

AU - De Angelis, Giulia

AU - Sanguinetti, Maurizio

AU - Kemper, Merel

AU - de Jong, Anniek E.E.

AU - Mohammadi, Sima

AU - Renaud, Valentine

AU - Kukavica-Ibrulj, Irena

AU - Potvin, Marianne

AU - Nguyen, Guillaume Q.

AU - Gauthier, Jeff

AU - Levesque, Roger C.

AU - Schmitt, Heike

AU - Severin, Juliëtte A.

PY - 2025/1/15

Y1 - 2025/1/15

N2 - Background/Objectives: The global spread of carbapenem-resistant Pseudomonas aeruginosa (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insufficient knowledge on the sources and transmission routes of CRPA. The aim of the SAMPAN study (“A Smart Surveillance Strategy for Carbapenem-resistant Pseudomonas aeruginosa”) is to develop a globally applicable surveillance strategy. Methods: First, an international cross-sectional study will be conducted to investigate CRPA in clinical and environmental settings in Rotterdam (The Netherlands), Rome (Italy), and Jakarta (Indonesia). Screening cultures and risk factor questionnaires will be taken from healthy individuals and patients upon hospital admission. Clinical CRPA isolates will also be included. Additionally, samples will be taken twice from wet hospital environments and monthly from the hospitals’ (drinking) water system, hospital and municipal wastewater treatment plants, and receiving rivers. Whole-genome sequencing will be performed to characterize CRPA isolates and determine the genetic relatedness among the isolates from different reservoirs. Findings from the cross-sectional study, combined with expert elicitation using a Delphi method, will serve as the input for the surveillance strategy. Conclusions: The SAMPAN study will provide a broader understanding of the sources and transmission routes of CRPA. Therewith, the development of a globally applicable smart surveillance strategy will be made possible, delivering information that is needed to guide actions against the spread of CRPA.

AB - Background/Objectives: The global spread of carbapenem-resistant Pseudomonas aeruginosa (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insufficient knowledge on the sources and transmission routes of CRPA. The aim of the SAMPAN study (“A Smart Surveillance Strategy for Carbapenem-resistant Pseudomonas aeruginosa”) is to develop a globally applicable surveillance strategy. Methods: First, an international cross-sectional study will be conducted to investigate CRPA in clinical and environmental settings in Rotterdam (The Netherlands), Rome (Italy), and Jakarta (Indonesia). Screening cultures and risk factor questionnaires will be taken from healthy individuals and patients upon hospital admission. Clinical CRPA isolates will also be included. Additionally, samples will be taken twice from wet hospital environments and monthly from the hospitals’ (drinking) water system, hospital and municipal wastewater treatment plants, and receiving rivers. Whole-genome sequencing will be performed to characterize CRPA isolates and determine the genetic relatedness among the isolates from different reservoirs. Findings from the cross-sectional study, combined with expert elicitation using a Delphi method, will serve as the input for the surveillance strategy. Conclusions: The SAMPAN study will provide a broader understanding of the sources and transmission routes of CRPA. Therewith, the development of a globally applicable smart surveillance strategy will be made possible, delivering information that is needed to guide actions against the spread of CRPA.

UR - http://www.scopus.com/inward/record.url?scp=85216229814&partnerID=8YFLogxK

U2 - 10.3390/antibiotics14010094

DO - 10.3390/antibiotics14010094

M3 - Article

AN - SCOPUS:85216229814

SN - 2079-6382

VL - 14

JO - Antibiotics

JF - Antibiotics

IS - 1

M1 - 94

ER -

Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa: Study Design and Methodology of the SAMPAN Study

Abstract

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