Sparse deconvolution of high-density super-resolution images

S Hugelier, Jacob Rooi, R Bernex, S Duwe, O Devos, M Sliwa, P Dedecker, Paul Eilers, C Ruckebusch

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Abstract

In wide-field super-resolution microscopy, investigating the nanoscale structure of cellular processes, and resolving fast dynamics and morphological changes in cells requires algorithms capable of working with a high-density of emissive fluorophores. Current deconvolution algorithms estimate fluorophore density by using representations of the signal that promote sparsity of the super-resolution images via an L-1-norm penalty. This penalty imposes a restriction on the sum of absolute values of the estimates of emitter brightness. By implementing an L-0-norm penalty - on the number of fluorophores rather than on their overall brightness - we present a penalized regression approach that can work at high-density and allows fast super-resolution imaging. We validated our approach on simulated images with densities up to 15 emitters per mu m(-2) and investigated total internal reflection fluorescence (TIRF) data of mitochondria in a HEK293-T cell labeled with DAKAP-Dronpa. We demonstrated super-resolution imaging of the dynamics with a resolution down to 55 nm and a 0.5 s time sampling.
Original languageUndefined/Unknown
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016

Research programs

  • EMC NIHES-01-66-01

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