Specific serology for emerging human coronaviruses by protein microarray

Chantal Reusken; H Mou; GJ Godeke; L (László) van den Hoek; B Meyer; MA Muller; Bart Haagmans; R de Sousa; N Schuurman; U Dittmer; P Rottier; Ab Osterhaus; C Drosten; BJ (Berend Jan) Bosch; Marion Koopmans

Specific serology for emerging human coronaviruses by protein microarray

Chantal Reusken, H Mou, GJ Godeke, L (László) van den Hoek, B Meyer, MA Muller, Bart Haagmans, R de Sousa, N Schuurman, U Dittmer, P Rottier, Ab Osterhaus, C Drosten, BJ (Berend Jan) Bosch, Marion Koopmans

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Abstract

We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

Original language	Undefined/Unknown
Pages (from-to)	2-8
Number of pages	7
Journal	Eurosurveillance
Volume	18
Issue number	14
Publication status	Published - 2013

Cite this

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title = "Specific serology for emerging human coronaviruses by protein microarray",

abstract = "We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.",

author = "Chantal Reusken and H Mou and GJ Godeke and {van den Hoek}, {L (L{\'a}szl{\'o})} and B Meyer and MA Muller and Bart Haagmans and {de Sousa}, R and N Schuurman and U Dittmer and P Rottier and Ab Osterhaus and C Drosten and Bosch, {BJ (Berend Jan)} and Marion Koopmans",

year = "2013",

language = "Undefined/Unknown",

volume = "18",

pages = "2--8",

journal = "Eurosurveillance",

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TY - JOUR

T1 - Specific serology for emerging human coronaviruses by protein microarray

AU - Reusken, Chantal

AU - Mou, H

AU - Godeke, GJ

AU - van den Hoek, L (László)

AU - Meyer, B

AU - Muller, MA

AU - Haagmans, Bart

AU - de Sousa, R

AU - Schuurman, N

AU - Dittmer, U

AU - Rottier, P

AU - Osterhaus, Ab

AU - Drosten, C

AU - Bosch, BJ (Berend Jan)

AU - Koopmans, Marion

PY - 2013

Y1 - 2013

N2 - We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

AB - We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

M3 - Article

SN - 1560-7917

VL - 18

SP - 2

EP - 8

JO - Eurosurveillance

JF - Eurosurveillance

IS - 14

ER -