Specific serology for emerging human coronaviruses by protein microarray

Chantal Reusken, H Mou, GJ Godeke, L (László) van den Hoek, B Meyer, MA Muller, Bart Haagmans, R de Sousa, N Schuurman, U Dittmer, P Rottier, Ab Osterhaus, C Drosten, BJ (Berend Jan) Bosch, Marion Koopmans

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Abstract

We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.
Original languageUndefined/Unknown
Pages (from-to)2-8
Number of pages7
JournalEurosurveillance
Volume18
Issue number14
Publication statusPublished - 2013

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