Spontaneous morphological changes by overexpression of the growth-associated protein B-50/GAP-43 in a PC12 cell line

H. B. Nielander; P. French; A. B. Oestreicher; W. H. Gispen; P. Schotman

doi:10.1016/0304-3940(93)90556-Z

Spontaneous morphological changes by overexpression of the growth-associated protein B-50/GAP-43 in a PC12 cell line

H. B. Nielander
, P. French
, A. B. Oestreicher
, W. H. Gispen
, P. Schotman^*

^*Corresponding author for this work

Brain Centre Rudolf Magnus

Research output: Contribution to journal › Article › Academic › peer-review

23 Citations (Scopus)

Abstract

In order to study the direct effects of B-50 on neural cell morphology, rat B-50 cDNA was transfected into a PC12 cell line (PC-B2) exhibiting neurite outgrowth independent of the expression of endogenous B-50. The morphological changes were visualized by confocal scanning laser microscopy using fluorescence labeling for B-50 and for F-actin. The transfected cells exhibited filopodia and/or blebs on the plasma membrane, containing most of the B-50 immunoreactivity. No spontaneous neurite outgrowth was observed. Following NGF treatment transfected and non-transfected PC-B2 cells extended F-actin positive filopodia and neurites with a striking colocalisation of B-50 and F-actin. Our data show that the presence of B-50 can influence cell surface morphology independent of the presence of NGF. The colocalisation of B-50 and F-actin in the filopodial protrusions but not in the blebs might be indicative for a role of B-50 in actin polymerization and depolymerization.

Original language	English
Pages (from-to)	46-50
Number of pages	5
Journal	Neuroscience Letters
Volume	162
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(93)90556-Z
Publication status	Published - 12 Nov 1993
Externally published	Yes

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title = "Spontaneous morphological changes by overexpression of the growth-associated protein B-50/GAP-43 in a PC12 cell line",

abstract = "In order to study the direct effects of B-50 on neural cell morphology, rat B-50 cDNA was transfected into a PC12 cell line (PC-B2) exhibiting neurite outgrowth independent of the expression of endogenous B-50. The morphological changes were visualized by confocal scanning laser microscopy using fluorescence labeling for B-50 and for F-actin. The transfected cells exhibited filopodia and/or blebs on the plasma membrane, containing most of the B-50 immunoreactivity. No spontaneous neurite outgrowth was observed. Following NGF treatment transfected and non-transfected PC-B2 cells extended F-actin positive filopodia and neurites with a striking colocalisation of B-50 and F-actin. Our data show that the presence of B-50 can influence cell surface morphology independent of the presence of NGF. The colocalisation of B-50 and F-actin in the filopodial protrusions but not in the blebs might be indicative for a role of B-50 in actin polymerization and depolymerization.",

author = "Nielander, \{H. B.\} and P. French and Oestreicher, \{A. B.\} and Gispen, \{W. H.\} and P. Schotman",

year = "1993",

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doi = "10.1016/0304-3940(93)90556-Z",

language = "English",

volume = "162",

pages = "46--50",

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T1 - Spontaneous morphological changes by overexpression of the growth-associated protein B-50/GAP-43 in a PC12 cell line

AU - Nielander, H. B.

AU - French, P.

AU - Oestreicher, A. B.

AU - Gispen, W. H.

AU - Schotman, P.

PY - 1993/11/12

Y1 - 1993/11/12

N2 - In order to study the direct effects of B-50 on neural cell morphology, rat B-50 cDNA was transfected into a PC12 cell line (PC-B2) exhibiting neurite outgrowth independent of the expression of endogenous B-50. The morphological changes were visualized by confocal scanning laser microscopy using fluorescence labeling for B-50 and for F-actin. The transfected cells exhibited filopodia and/or blebs on the plasma membrane, containing most of the B-50 immunoreactivity. No spontaneous neurite outgrowth was observed. Following NGF treatment transfected and non-transfected PC-B2 cells extended F-actin positive filopodia and neurites with a striking colocalisation of B-50 and F-actin. Our data show that the presence of B-50 can influence cell surface morphology independent of the presence of NGF. The colocalisation of B-50 and F-actin in the filopodial protrusions but not in the blebs might be indicative for a role of B-50 in actin polymerization and depolymerization.

AB - In order to study the direct effects of B-50 on neural cell morphology, rat B-50 cDNA was transfected into a PC12 cell line (PC-B2) exhibiting neurite outgrowth independent of the expression of endogenous B-50. The morphological changes were visualized by confocal scanning laser microscopy using fluorescence labeling for B-50 and for F-actin. The transfected cells exhibited filopodia and/or blebs on the plasma membrane, containing most of the B-50 immunoreactivity. No spontaneous neurite outgrowth was observed. Following NGF treatment transfected and non-transfected PC-B2 cells extended F-actin positive filopodia and neurites with a striking colocalisation of B-50 and F-actin. Our data show that the presence of B-50 can influence cell surface morphology independent of the presence of NGF. The colocalisation of B-50 and F-actin in the filopodial protrusions but not in the blebs might be indicative for a role of B-50 in actin polymerization and depolymerization.

UR - https://www.scopus.com/pages/publications/0027451304

U2 - 10.1016/0304-3940(93)90556-Z

DO - 10.1016/0304-3940(93)90556-Z

M3 - Article

C2 - 8121634

AN - SCOPUS:0027451304

SN - 0304-3940

VL - 162

SP - 46

EP - 50

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Spontaneous morphological changes by overexpression of the growth-associated protein B-50/GAP-43 in a PC12 cell line

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