Staphylococcus aureus RN6390 replicates and induces apoptosis in a pulmonary epithelial cell line

B. C. Kahl, M. Goulian, W. Van Wamel, M. Herrmann, S.M. Simon, G. Kaplan, G. Peters, A. L. Cheung*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

179 Citations (Scopus)

Abstract

Staphylococcus aureus frequently colonizes the airways of patients with compromised airway defenses (e.g., cystic fibrosis [CF] patients) for extended periods. Persistent and relapsing infections may be related to live S. aureus bacteria actively residing inside epithelial cells. In this study, we infected a respiratory epithelial cell line, which was derived from a CF patient, with S. aureus RN6390. Internalization of S. aureus was found to be time and dose dependent and could be blocked by cytochalasin D. Transmission electron microscopy revealed that internalized bacteria resided within endocytic vacuoles without any evidence of lysosomal fusion in a 24-h period. The results of internalization experiments and time-lapse fluorescence microscopy of epithelial cells infected with green fluorescent S. aureus indicate that, after an initial lag period of 7 to 9 h, intracellular bacteria began to replicate, with three to five divisions in a 24-h period, leading to apoptosis of infected cells. Induction of apoptosis required bacterial internalization and is associated with intracellular replication. The slow and gradual replication of S. aureus inside epithelial cells hints at the role of host factors or signals in bacterial growth and further suggests possible cross talk between host cells and S. aureus.

Original languageEnglish
Pages (from-to)5385-5392
Number of pages8
JournalInfection and Immunity
Volume68
Issue number9
DOIs
Publication statusPublished - 2000
Externally publishedYes

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