Staphylococcus epidermidis originating from titanium implants infects surrounding tissue and immune cells

M Riool, L Boer, V Jaspers, CM van der Loos, Willem van Wamel, G Wu, PHS Kwakman, SAJ Zaat

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69 Citations (Scopus)

Abstract

Infection is a major cause of failure of inserted or implanted biomedical devices (biomaterials). During surgery, bacteria may adhere to the implant, initiating biofilm formation. Bacteria are also observed in and recultured from the tissue surrounding implants, and may even reside inside host cells. Whether these bacteria originate from biofilms is not known. Therefore, we investigated the fate of Staphylococcus epidermidis inoculated on the surface of implants as adherent planktonic cells or as a biofilm in mouse experimental biomaterial-associated infection. In order to discriminate the challenge strain from potential contaminating mouse microflora, we constructed a fully virulent green fluorescent S. epidermidis strain. S. epidermidis injected along subcutaneous titanium implants, pre-seeded on the implants or pre-grown as biofilm, were retrieved from the implants as well as the surrounding tissue in all cases after 4 days, and in histology bacteria were observed in the tissue co-localizing with macrophages. Thus, bacteria adherent to or in a biofilm on the implant are a potential source of infection of the surrounding tissue, and antimicrobial strategies should prevent both biofilm formation and tissue colonization. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)5202-5212
Number of pages11
JournalActa Biomaterialia
Volume10
Issue number12
DOIs
Publication statusPublished - 2014

Research programs

  • EMC MM-04-28-01

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