Streptococcus pneumoniae exposure is associated with human metapneumovirus seroconversion and increased susceptibility to in vitro HMPV infection

Nelianne Verkaik, Tien Nguyen, Corne de Vogel, Henriette Moll, Henri Verbrugh, Vincent Jaddoe, Bert Hofman, Willem van Wamel, BG van den Hoogen, Luna Offerman, Martin Ludlow, L de Witte, Ab Osterhaus, Alex Belkum, Rik de Swart

Research output: Contribution to journalArticleAcademic

38 Citations (Scopus)

Abstract

It remains largely unknown which factors determine the clinical outcome of human metapneumovirus (HMPV) infections. The aim of the present study was to analyse whether exposure to bacterial pathogens can influence HMPV infections. From 57 children, serum samples and colonization data for Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and Streptococcus pneumoniae were collected at 1.5, 6, 14 and 24 months of age. Seroconversion rates to HMPV were determined and related to bacterial carriage. Frequent nasopharyngeal carriage (=2 times in the first 2 years of life) of S. pneumoniae, but not of the other three pathogens, was associated with increased seroconversion rates of infants to HMPV at the age of 2 years (frequently vs. less exposed, 93% vs. 59%; p <0.05). Subsequently, the susceptibility of well-differentiated normal human bronchial epithelial cells (wd-NHBE) pre-incubated with bacterial pathogens to in vitro HMPV infection was evaluated. Pre-incubation of wd-NHBE with S. pneumoniae resulted in increased susceptibility to infection with HMPV-enhanced green fluorescent protein (EGFP), as determined by enumeration of EGFP-positive cells. This was not the case for cells pre-incubated with H. influenzae, M. catarrhalis on S. aureus. We conclude that exposure to S. pneumoniae can modulate HMPV infection.
Original languageUndefined/Unknown
Pages (from-to)1840-1844
Number of pages5
JournalClinical Microbiology and Infection
Volume17
Issue number12
DOIs
Publication statusPublished - 2011

Cite this