Study of rat skeletal muscle activation by PET/CT and [11C]acetate

Sara Trombella, David Garcia, Didier J. Colin, Stephane Germain, Yann Seimbille, Osman Ratib

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Purpose: The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the skeletal muscle of the rat hind limb by [11C]acetate PET/CT. Methods: One-leg contractions were induced in Wistar rats by electrostimulation of the VL muscle motor point. After 15 minutes of muscle contractions [11C]acetate was injected and PET/CT of the rat hind limbs was acquired. The resting leg was used as a control reference. The perfusion index F was calculated for the target muscles (exercised and control) and correlated to the corresponding SUVs. For both F and the SUV, the ratio of the value extracted for the active muscle to the one derived for the muscle at rest was computed (respectively, FE/FR and SUVE/SUVR). Results: For the experimental dataset, a strong correlation (R2 = 0.97, P = 0.01) between FE/FR and SUVE/SUVR was observed. The ratio FE/FR resulted to be higher than the ratio SUVE/SUVR (average values: 2.1 ± 0.7 and 1.19 ± 0.08, respectively). Conclusion: Preliminary results indicate that both kinetic modeling and the SUV can detect changes in muscles between rest and exercise conditions, when associated to [11C]acetate PET/CT.

Original languageEnglish
Pages (from-to)1427-1430
Number of pages4
JournalProceedings - International Symposium on Biomedical Imaging
DOIs
Publication statusPublished - 21 Jul 2015
Externally publishedYes
Event12th IEEE International Symposium on Biomedical Imaging, ISBI 2015 - Brooklyn, United States
Duration: 16 Apr 201519 Apr 2015

Bibliographical note

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© 2015 IEEE.

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