Study of rat skeletal muscle activation by PET/CT and [11C]acetate

Sara Trombella; David Garcia; Didier J. Colin; Stephane Germain; Yann Seimbille; Osman Ratib

doi:10.1109/ISBI.2015.7164144

Study of rat skeletal muscle activation by PET/CT and [¹¹C]acetate

Sara Trombella
, David Garcia
, Didier J. Colin
, Stephane Germain
, Yann Seimbille
, Osman Ratib

University Hospital of Geneva
University of Geneva
Centre for Biomedical Imaging

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Purpose: The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the skeletal muscle of the rat hind limb by [¹¹C]acetate PET/CT. Methods: One-leg contractions were induced in Wistar rats by electrostimulation of the VL muscle motor point. After 15 minutes of muscle contractions [¹¹C]acetate was injected and PET/CT of the rat hind limbs was acquired. The resting leg was used as a control reference. The perfusion index F was calculated for the target muscles (exercised and control) and correlated to the corresponding SUVs. For both F and the SUV, the ratio of the value extracted for the active muscle to the one derived for the muscle at rest was computed (respectively, F_E/F_R and SUV_E/SUV_R). Results: For the experimental dataset, a strong correlation (R² = 0.97, P = 0.01) between F_E/F_R and SUV_E/SUV_R was observed. The ratio F_E/F_R resulted to be higher than the ratio SUV_E/SUV_R (average values: 2.1 ± 0.7 and 1.19 ± 0.08, respectively). Conclusion: Preliminary results indicate that both kinetic modeling and the SUV can detect changes in muscles between rest and exercise conditions, when associated to [¹¹C]acetate PET/CT.

Original language	English
Pages (from-to)	1427-1430
Number of pages	4
Journal	Proceedings - International Symposium on Biomedical Imaging
DOIs	https://doi.org/10.1109/ISBI.2015.7164144
Publication status	Published - 21 Jul 2015
Externally published	Yes
Event	12th IEEE International Symposium on Biomedical Imaging, ISBI 2015 - Brooklyn, United States Duration: 16 Apr 2015 → 19 Apr 2015

Bibliographical note

Publisher Copyright:
© 2015 IEEE.

Access to Document

10.1109/ISBI.2015.7164144

Cite this

@article{cf8f214708b840a3897aceb3147b776d,

title = "Study of rat skeletal muscle activation by PET/CT and [11C]acetate",

abstract = "Purpose: The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the skeletal muscle of the rat hind limb by [11C]acetate PET/CT. Methods: One-leg contractions were induced in Wistar rats by electrostimulation of the VL muscle motor point. After 15 minutes of muscle contractions [11C]acetate was injected and PET/CT of the rat hind limbs was acquired. The resting leg was used as a control reference. The perfusion index F was calculated for the target muscles (exercised and control) and correlated to the corresponding SUVs. For both F and the SUV, the ratio of the value extracted for the active muscle to the one derived for the muscle at rest was computed (respectively, FE/FR and SUVE/SUVR). Results: For the experimental dataset, a strong correlation (R2 = 0.97, P = 0.01) between FE/FR and SUVE/SUVR was observed. The ratio FE/FR resulted to be higher than the ratio SUVE/SUVR (average values: 2.1 ± 0.7 and 1.19 ± 0.08, respectively). Conclusion: Preliminary results indicate that both kinetic modeling and the SUV can detect changes in muscles between rest and exercise conditions, when associated to [11C]acetate PET/CT.",

author = "Sara Trombella and David Garcia and Colin, \{Didier J.\} and Stephane Germain and Yann Seimbille and Osman Ratib",

note = "Publisher Copyright: {\textcopyright} 2015 IEEE.; 12th IEEE International Symposium on Biomedical Imaging, ISBI 2015 ; Conference date: 16-04-2015 Through 19-04-2015",

year = "2015",

month = jul,

day = "21",

doi = "10.1109/ISBI.2015.7164144",

language = "English",

pages = "1427--1430",

}

TY - JOUR

T1 - Study of rat skeletal muscle activation by PET/CT and [11C]acetate

AU - Trombella, Sara

AU - Garcia, David

AU - Colin, Didier J.

AU - Germain, Stephane

AU - Seimbille, Yann

AU - Ratib, Osman

PY - 2015/7/21

Y1 - 2015/7/21

N2 - Purpose: The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the skeletal muscle of the rat hind limb by [11C]acetate PET/CT. Methods: One-leg contractions were induced in Wistar rats by electrostimulation of the VL muscle motor point. After 15 minutes of muscle contractions [11C]acetate was injected and PET/CT of the rat hind limbs was acquired. The resting leg was used as a control reference. The perfusion index F was calculated for the target muscles (exercised and control) and correlated to the corresponding SUVs. For both F and the SUV, the ratio of the value extracted for the active muscle to the one derived for the muscle at rest was computed (respectively, FE/FR and SUVE/SUVR). Results: For the experimental dataset, a strong correlation (R2 = 0.97, P = 0.01) between FE/FR and SUVE/SUVR was observed. The ratio FE/FR resulted to be higher than the ratio SUVE/SUVR (average values: 2.1 ± 0.7 and 1.19 ± 0.08, respectively). Conclusion: Preliminary results indicate that both kinetic modeling and the SUV can detect changes in muscles between rest and exercise conditions, when associated to [11C]acetate PET/CT.

AB - Purpose: The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the skeletal muscle of the rat hind limb by [11C]acetate PET/CT. Methods: One-leg contractions were induced in Wistar rats by electrostimulation of the VL muscle motor point. After 15 minutes of muscle contractions [11C]acetate was injected and PET/CT of the rat hind limbs was acquired. The resting leg was used as a control reference. The perfusion index F was calculated for the target muscles (exercised and control) and correlated to the corresponding SUVs. For both F and the SUV, the ratio of the value extracted for the active muscle to the one derived for the muscle at rest was computed (respectively, FE/FR and SUVE/SUVR). Results: For the experimental dataset, a strong correlation (R2 = 0.97, P = 0.01) between FE/FR and SUVE/SUVR was observed. The ratio FE/FR resulted to be higher than the ratio SUVE/SUVR (average values: 2.1 ± 0.7 and 1.19 ± 0.08, respectively). Conclusion: Preliminary results indicate that both kinetic modeling and the SUV can detect changes in muscles between rest and exercise conditions, when associated to [11C]acetate PET/CT.

UR - https://www.scopus.com/pages/publications/84944329649

U2 - 10.1109/ISBI.2015.7164144

DO - 10.1109/ISBI.2015.7164144

M3 - Article

AN - SCOPUS:84944329649

SN - 1945-7928

SP - 1427

EP - 1430

JO - Proceedings - International Symposium on Biomedical Imaging

JF - Proceedings - International Symposium on Biomedical Imaging

T2 - 12th IEEE International Symposium on Biomedical Imaging, ISBI 2015

Y2 - 16 April 2015 through 19 April 2015

ER -

Study of rat skeletal muscle activation by PET/CT and [¹¹C]acetate

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this