Surface contact of bound targeted microbubbles

Klazina Kooiman, Tom J.A. Kokhuis, Ilya Skachkov, Johan G. Bosch, Antonius F.W. Van Der Steen, Wiggert A. Van Cappellen, Nico De Jong

Research output: Chapter/Conference proceedingConference proceedingAcademicpeer-review

4 Citations (Scopus)

Abstract

For molecular imaging using ultrasound contrast agents, targeted microbubbles are designed with specific ligands linked to the coated shell. Research is ongoing to determine the binding force of targeted microbubbles and to distinguish bound from unbound targeted microbubbles using ultrasound. For this, the actual surface of the targeted microbubbles that binds to a pathology is important. This study focuses on determining the surface contact of bound targeted microbubbles by fluorescence microscopy. Biotinylated lipid-coated microbubbles (3-7 μm in diameter) with either DSPC or DPPC as the main lipid were targeted to a streptavidin-coated surface. The binding area of targeted microbubbles was found to be 6 ± 4% of the total microbubble surface for microbubbles with DSPC as the main lipid (n=22) and 11 ± 4% for microbubbles with DPPC as the main lipid (n=24). The difference can be explained by the heterogeneous distribution of the ligand for DSPC microbubbles whereas the ligand is homogeneously distributed for DPPC microbubbles. These findings can be used to improve the binding of targeted microbubbles and for the ongoing research to distinguish bound from unbound microbubbles.

Original languageEnglish
Title of host publication2012 IEEE International Ultrasonics Symposium, IUS 2012
Pages2161-2163
Number of pages3
DOIs
Publication statusPublished - 2012
Event2012 IEEE International Ultrasonics Symposium, IUS 2012 - Dresden, Germany
Duration: 7 Oct 201210 Oct 2012

Publication series

SeriesIEEE International Ultrasonics Symposium, IUS
ISSN1948-5719

Conference

Conference2012 IEEE International Ultrasonics Symposium, IUS 2012
Country/TerritoryGermany
CityDresden
Period7/10/1210/10/12

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