TY - GEN
T1 - Surface contact of bound targeted microbubbles
AU - Kooiman, Klazina
AU - Kokhuis, Tom J.A.
AU - Skachkov, Ilya
AU - Bosch, Johan G.
AU - Van Der Steen, Antonius F.W.
AU - Van Cappellen, Wiggert A.
AU - De Jong, Nico
PY - 2012
Y1 - 2012
N2 - For molecular imaging using ultrasound contrast agents, targeted microbubbles are designed with specific ligands linked to the coated shell. Research is ongoing to determine the binding force of targeted microbubbles and to distinguish bound from unbound targeted microbubbles using ultrasound. For this, the actual surface of the targeted microbubbles that binds to a pathology is important. This study focuses on determining the surface contact of bound targeted microbubbles by fluorescence microscopy. Biotinylated lipid-coated microbubbles (3-7 μm in diameter) with either DSPC or DPPC as the main lipid were targeted to a streptavidin-coated surface. The binding area of targeted microbubbles was found to be 6 ± 4% of the total microbubble surface for microbubbles with DSPC as the main lipid (n=22) and 11 ± 4% for microbubbles with DPPC as the main lipid (n=24). The difference can be explained by the heterogeneous distribution of the ligand for DSPC microbubbles whereas the ligand is homogeneously distributed for DPPC microbubbles. These findings can be used to improve the binding of targeted microbubbles and for the ongoing research to distinguish bound from unbound microbubbles.
AB - For molecular imaging using ultrasound contrast agents, targeted microbubbles are designed with specific ligands linked to the coated shell. Research is ongoing to determine the binding force of targeted microbubbles and to distinguish bound from unbound targeted microbubbles using ultrasound. For this, the actual surface of the targeted microbubbles that binds to a pathology is important. This study focuses on determining the surface contact of bound targeted microbubbles by fluorescence microscopy. Biotinylated lipid-coated microbubbles (3-7 μm in diameter) with either DSPC or DPPC as the main lipid were targeted to a streptavidin-coated surface. The binding area of targeted microbubbles was found to be 6 ± 4% of the total microbubble surface for microbubbles with DSPC as the main lipid (n=22) and 11 ± 4% for microbubbles with DPPC as the main lipid (n=24). The difference can be explained by the heterogeneous distribution of the ligand for DSPC microbubbles whereas the ligand is homogeneously distributed for DPPC microbubbles. These findings can be used to improve the binding of targeted microbubbles and for the ongoing research to distinguish bound from unbound microbubbles.
UR - http://www.scopus.com/inward/record.url?scp=84882428148&partnerID=8YFLogxK
U2 - 10.1109/ULTSYM.2012.0539
DO - 10.1109/ULTSYM.2012.0539
M3 - Conference proceeding
AN - SCOPUS:84882428148
SN - 9781467345613
T3 - IEEE International Ultrasonics Symposium, IUS
SP - 2161
EP - 2163
BT - 2012 IEEE International Ultrasonics Symposium, IUS 2012
T2 - 2012 IEEE International Ultrasonics Symposium, IUS 2012
Y2 - 7 October 2012 through 10 October 2012
ER -