Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method

Abraham J. Paredes-Fuentes; Clara Oliva; Raquel Montero; Patricia Alcaide; George J.G. Ruijter; Judit García-Villoria; Pedro Ruiz-Sala; Rafael Artuch

doi:10.3390/antiox11030528

Technical Aspects of Coenzyme Q₁₀ Analysis: Validation of a New HPLC-ED Method

Abraham J. Paredes-Fuentes^*, Clara Oliva, Raquel Montero, Patricia Alcaide, George J.G. Ruijter, Judit García-Villoria, Pedro Ruiz-Sala, Rafael Artuch

^*Corresponding author for this work

Clinical Genetics

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

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Abstract

The biochemical measurement of the CoQ status in different tissues can be performed using HPLC with electrochemical detection (ED). Because the production of the electrochemical cells used with the Coulochem series detectors was discontinued, we aimed to standardize a new HPLC-ED method with new equipment. We report all technical aspects, troubleshooting and its performance in different biological samples, including plasma, skeletal muscle homogenates, urine and cultured skin fibroblasts. Analytical variables (intra-and inter-assay precision, linearity, analytical measurement range, limit of quantification, limit of detection and accuracy) were validated in calibrators and plasma samples and displayed adequate results. The comparison of the results of a new ERNDIM external quality control (EQC) scheme for the plasma CoQ determination between HPLC-ED (Lab 1) and LC-MS/MS (Lab 2) methods shows that the results of the latter were slightly higher in most cases, although a good consistency was generally observed. In conclusion, the new method reported here showed a good analytical performance. The global quality of the EQC scheme results among different participants can be improved with the contribution of more laboratories.

Original language	English
Article number	528
Journal	Antioxidants
Volume	11
Issue number	3
DOIs	https://doi.org/10.3390/antiox11030528
Publication status	Published - 10 Mar 2022

Bibliographical note

Funding Information:
This work has been funded by the ?Instituto de Salud Carlos III? (PI20/00340) (co-funded by European Regional Development Fund ?A way to make Europe?), FEDER 2014?2020, and ?Asociaci?n de Enfermos de Patolog?as Mitocondriales (AEPMI)?. CIBERER is an initiative from the ?Instituto de Salud Carlos III?. A.J.P.-F. held a grant from the ?Instituto de Salud Carlos III? (FI18/00253) and from the ?Asociaci?n de Enfermos de Patolog?as Mitocondriales (AEPMI)/Fundaci?n Ana Carolina Diez Mahou?.

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© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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title = "Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method",

abstract = "The biochemical measurement of the CoQ status in different tissues can be performed using HPLC with electrochemical detection (ED). Because the production of the electrochemical cells used with the Coulochem series detectors was discontinued, we aimed to standardize a new HPLC-ED method with new equipment. We report all technical aspects, troubleshooting and its performance in different biological samples, including plasma, skeletal muscle homogenates, urine and cultured skin fibroblasts. Analytical variables (intra-and inter-assay precision, linearity, analytical measurement range, limit of quantification, limit of detection and accuracy) were validated in calibrators and plasma samples and displayed adequate results. The comparison of the results of a new ERNDIM external quality control (EQC) scheme for the plasma CoQ determination between HPLC-ED (Lab 1) and LC-MS/MS (Lab 2) methods shows that the results of the latter were slightly higher in most cases, although a good consistency was generally observed. In conclusion, the new method reported here showed a good analytical performance. The global quality of the EQC scheme results among different participants can be improved with the contribution of more laboratories.",

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note = "Funding Information: This work has been funded by the ?Instituto de Salud Carlos III? (PI20/00340) (co-funded by European Regional Development Fund ?A way to make Europe?), FEDER 2014?2020, and ?Asociaci?n de Enfermos de Patolog?as Mitocondriales (AEPMI)?. CIBERER is an initiative from the ?Instituto de Salud Carlos III?. A.J.P.-F. held a grant from the ?Instituto de Salud Carlos III? (FI18/00253) and from the ?Asociaci?n de Enfermos de Patolog?as Mitocondriales (AEPMI)/Fundaci?n Ana Carolina Diez Mahou?. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - García-Villoria, Judit

AU - Ruiz-Sala, Pedro

AU - Artuch, Rafael

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PY - 2022/3/10

Y1 - 2022/3/10

N2 - The biochemical measurement of the CoQ status in different tissues can be performed using HPLC with electrochemical detection (ED). Because the production of the electrochemical cells used with the Coulochem series detectors was discontinued, we aimed to standardize a new HPLC-ED method with new equipment. We report all technical aspects, troubleshooting and its performance in different biological samples, including plasma, skeletal muscle homogenates, urine and cultured skin fibroblasts. Analytical variables (intra-and inter-assay precision, linearity, analytical measurement range, limit of quantification, limit of detection and accuracy) were validated in calibrators and plasma samples and displayed adequate results. The comparison of the results of a new ERNDIM external quality control (EQC) scheme for the plasma CoQ determination between HPLC-ED (Lab 1) and LC-MS/MS (Lab 2) methods shows that the results of the latter were slightly higher in most cases, although a good consistency was generally observed. In conclusion, the new method reported here showed a good analytical performance. The global quality of the EQC scheme results among different participants can be improved with the contribution of more laboratories.

AB - The biochemical measurement of the CoQ status in different tissues can be performed using HPLC with electrochemical detection (ED). Because the production of the electrochemical cells used with the Coulochem series detectors was discontinued, we aimed to standardize a new HPLC-ED method with new equipment. We report all technical aspects, troubleshooting and its performance in different biological samples, including plasma, skeletal muscle homogenates, urine and cultured skin fibroblasts. Analytical variables (intra-and inter-assay precision, linearity, analytical measurement range, limit of quantification, limit of detection and accuracy) were validated in calibrators and plasma samples and displayed adequate results. The comparison of the results of a new ERNDIM external quality control (EQC) scheme for the plasma CoQ determination between HPLC-ED (Lab 1) and LC-MS/MS (Lab 2) methods shows that the results of the latter were slightly higher in most cases, although a good consistency was generally observed. In conclusion, the new method reported here showed a good analytical performance. The global quality of the EQC scheme results among different participants can be improved with the contribution of more laboratories.

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Technical Aspects of Coenzyme Q₁₀ Analysis: Validation of a New HPLC-ED Method

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