TY - JOUR
T1 - The C-terminal domain of the adenomatous polyposis coli (Apc) protein is involved in thyroid morphogenesis and function
AU - Yokoyama, A
AU - Nomura, R
AU - Kurosumi, M
AU - Shimomura, A
AU - Onouchi, T
AU - Iizuka-Kogo, A
AU - Smits, Ron
AU - Oda, N
AU - Fodde, Riccardo
AU - Itoh, M
AU - Senda, T
PY - 2011
Y1 - 2011
N2 - Adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of Apc, we have investigated Apc (1638T/1638T) mice, which express a truncated Apc that lacks the C-terminal domain. Apc (1638T/1638T) mice are tumor free and exhibit growth retardation. In the present study, we analyzed the morphology and functions of the thyroid gland in Apc (1638T/1638T) mice. There was no significant difference in the basal concentration of serum thyroid hormones between Apc (1638T/1638T) and Apc (+/+) mice. Thyroid follicle size was significantly larger in Apc (1638T/1638T) mice than in Apc (+/+) mice. The extent of serum T4 elevation following exogenous thyroid-stimulating hormone (TSH) injection was lower in Apc (1638T/1638T) mice than in Apc (+/+) mice. TSH also induced a greater reduction in thyroid follicle size in Apc (1638T/1638T) mice than in Apc (+/+) mice. Analyses using immunohistochemistry and electron microscopy indicated that follicular epithelial cells in Apc (1638T/1638T) mice had an enlarged rough endoplasmic reticulum of irregular shape. These results suggest that the C-terminal domain of Apc is involved in thyroid morphology and function.
AB - Adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of Apc, we have investigated Apc (1638T/1638T) mice, which express a truncated Apc that lacks the C-terminal domain. Apc (1638T/1638T) mice are tumor free and exhibit growth retardation. In the present study, we analyzed the morphology and functions of the thyroid gland in Apc (1638T/1638T) mice. There was no significant difference in the basal concentration of serum thyroid hormones between Apc (1638T/1638T) and Apc (+/+) mice. Thyroid follicle size was significantly larger in Apc (1638T/1638T) mice than in Apc (+/+) mice. The extent of serum T4 elevation following exogenous thyroid-stimulating hormone (TSH) injection was lower in Apc (1638T/1638T) mice than in Apc (+/+) mice. TSH also induced a greater reduction in thyroid follicle size in Apc (1638T/1638T) mice than in Apc (+/+) mice. Analyses using immunohistochemistry and electron microscopy indicated that follicular epithelial cells in Apc (1638T/1638T) mice had an enlarged rough endoplasmic reticulum of irregular shape. These results suggest that the C-terminal domain of Apc is involved in thyroid morphology and function.
U2 - 10.1007/s00795-010-0529-9
DO - 10.1007/s00795-010-0529-9
M3 - Article
C2 - 22179183
SN - 1860-1480
VL - 44
SP - 207
EP - 212
JO - Medical Molecular Morphology
JF - Medical Molecular Morphology
IS - 4
ER -