The cross talk between pathways in the repair of 8-oxo-7,8-dihydroguanine in mouse and human cells

E Parlanti, M D'Errico, P Degan, A Calcagnile, A Zijno, Ingrid van der Pluijm, Bert van der Horst, DSF Biard, E Dogliotti

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Although oxidatively damaged DNA is repaired primarily via the base excision repair (BER) pathway, it is now evident that multiple subpathways are needed. Yet, their relative contributions and coordination are still unclear. Here, mouse embryo fibroblasts (MEFs) from selected nucleotide excision repair (NER) and/or BER mouse mutants with severe (Csb(m/m)/Xpa(-/-) and Csb(m/m)/Xpc(-/-)), mild (Csb(m/m)), or no progeria (X7a(-/-), Xpc(-/-), Ogg1(-/-), Csbm/m/Ogg1(-/-)) or wild-type phenotype were exposed to an oxidizing agent, potassium bromate, and genomic 8-oxo-7,8-dihydroguanine (8-oxoGua) levels were measured by HPLC-ED. The same oxidized DNA base was measured in NER/BER-defective human cell lines obtained after transfection with replicative plasmids encoding siRNA targeting DNA repair genes. We show that both BER and NER factors contribute to the repair of 8-oxoGua, although to different extents, and that the repair profiles are similar in human compared to mouse cells. The BER DNA glycosylase OGG1 dominates 8-oxoGua repair, whereas NER (XPC, XPA) and transcription-coupled repair proteins (CSB and CSA) are similar, but minor contributors. The comparison of DNA oxidation levels in double versus single defective MEFs indicates increased oxidatively damaged DNA only when both CSB a id XPC/XPA are defective, indicating that these proteins operate in different pathways. Moreover, vie provide the first evidence of an involvement of XPA in the control of oxidatively damaged DNA in human primary cells. (C) 2012 Elsevier Inc. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)2171-2177
Number of pages7
JournalFree Radical Biology and Medicine
Issue number11
Publication statusPublished - 2012

Research programs

  • EMC COEUR-09
  • EMC MGC-01-12-03

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