TY - JOUR
T1 - The effect of RGD-targeted and non-targeted liposomal Galbanic acid on the therapeutic efficacy of pegylated liposomal doxorubicin
T2 - From liposomal preparation to in-vivo studies
AU - Nik, Maryam Ebrahimi
AU - Jaafari, Mahmoud Reza
AU - Mashreghi, Mohammad
AU - Nikoofal-Sahlabadi, Sara
AU - Amin, Mohamadreza
AU - Sadeghnia, Hamid Reza
AU - Iranshahi, Mehrdad
AU - Navashenaq, Jamshid Gholizadeh
AU - Malaekeh-Nikouei, Bizhan
N1 - Copyright © 2021 Elsevier B.V. All rights reserved.
PY - 2021/7/15
Y1 - 2021/7/15
N2 - The anti-cancer therapeutic application of Galbanic acid (Gba) as a strong antiangiogenic sesquiterpene coumarin has been limited due to its low water solubility. This issue necessitates developing new liposomal formulations for the efficient delivery of Gba in vivo. In this study, various liposomal formulations were prepared by a thin-film hydration method, and Gba was incorporated into the liposomal bilayers, which consequently increased its release profile compared to formulations in our previous study prepared by remote loading methods. The most stable formulation with desired properties was selected and decorated with RGD peptide (cyclo [Arg-Gly-Asp-D-Tyr-Cys]) to target tumor vasculature actively. The fluorescently-labeled model liposomes showed that the targeting could improve the receptor-mediated endocytosis of the liposomes higher than those prepared in our previous study in vitro in human umbilical vein endothelial cells (HUVECs), which was confirmed by chicken chorioallantoic membrane angiogenesis (CAM) model in vivo. Although not significant, it also could increase the accumulation of liposomes in colon tumors. In BALB/c mice bearing colon cancer, not only non-targeted Gba liposomes but also even RGD-targeted ones combinatorial therapy with pegylated liposomal doxorubicin could improve the anti-tumor efficacy as compared to their monotherapy. These outcomes have strong consequences for cancer therapy.
AB - The anti-cancer therapeutic application of Galbanic acid (Gba) as a strong antiangiogenic sesquiterpene coumarin has been limited due to its low water solubility. This issue necessitates developing new liposomal formulations for the efficient delivery of Gba in vivo. In this study, various liposomal formulations were prepared by a thin-film hydration method, and Gba was incorporated into the liposomal bilayers, which consequently increased its release profile compared to formulations in our previous study prepared by remote loading methods. The most stable formulation with desired properties was selected and decorated with RGD peptide (cyclo [Arg-Gly-Asp-D-Tyr-Cys]) to target tumor vasculature actively. The fluorescently-labeled model liposomes showed that the targeting could improve the receptor-mediated endocytosis of the liposomes higher than those prepared in our previous study in vitro in human umbilical vein endothelial cells (HUVECs), which was confirmed by chicken chorioallantoic membrane angiogenesis (CAM) model in vivo. Although not significant, it also could increase the accumulation of liposomes in colon tumors. In BALB/c mice bearing colon cancer, not only non-targeted Gba liposomes but also even RGD-targeted ones combinatorial therapy with pegylated liposomal doxorubicin could improve the anti-tumor efficacy as compared to their monotherapy. These outcomes have strong consequences for cancer therapy.
UR - http://www.scopus.com/inward/record.url?scp=85107629920&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2021.120710
DO - 10.1016/j.ijpharm.2021.120710
M3 - Article
C2 - 34019972
AN - SCOPUS:85107629920
SN - 0378-5173
VL - 604
SP - 120710
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
M1 - 120710
ER -