The gut brain in a dish: Murine primary enteric nervous system cell cultures

Simone L. Schonkeren; Tara T. Küthe; Musa Idris; Ana C. Bon-Frauches; Werend Boesmans; Veerle Melotte

doi:10.1111/nmo.14215

The gut brain in a dish: Murine primary enteric nervous system cell cultures

Simone L. Schonkeren^*, Tara T. Küthe^*, Musa Idris, Ana C. Bon-Frauches, Werend Boesmans, Veerle Melotte

^*Corresponding author for this work

Clinical Genetics

Research output: Contribution to journal › Review article › Academic › peer-review

7 Citations (Scopus)

82 Downloads (Pure)

Abstract

Background: The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease.

Purpose: This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.

Original language	English
Article number	e14215
Journal	Neurogastroenterology and Motility
Volume	34
Issue number	2
Early online date	8 Jul 2021
DOIs	https://doi.org/10.1111/nmo.14215
Publication status	Published - Feb 2022

Bibliographical note

Funding Information:
This research was funded by The Netherlands Organisation for Scientific Research (NWO) Veni grant, grant number 016.186.124, VIDI grant, grant number 016.196.367, and HESTIA grant, grant number 1154.18.045, as well as by a grant from the Research Foundation Flanders (FWO), grant number G036320N

Publisher Copyright:
© 2021 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd.

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10.1111/nmo.14215Licence: CC BY-NC-ND

Neurogastroenterology Motil - 2021 - Schonkeren - The gut brain in a dish Murine primary enteric nervous system cellFinal published version, 1.26 MBLicence: CC BY-NC-ND

Cite this

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abstract = "Background: The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease. Purpose: This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.",

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AU - Boesmans, Werend

AU - Melotte, Veerle

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N2 - Background: The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease. Purpose: This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.

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The gut brain in a dish: Murine primary enteric nervous system cell cultures

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Bibliographical note

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