Background: The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease.
Purpose: This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.
Bibliographical noteFunding Information:
This research was funded by The Netherlands Organisation for Scientific Research (NWO) Veni grant, grant number 016.186.124, VIDI grant, grant number 016.196.367, and HESTIA grant, grant number 1154.18.045, as well as by a grant from the Research Foundation Flanders (FWO), grant number G036320N
© 2021 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd.