The miR-15a-miR-16-1 Locus is Homozygously Deleted in a Subset of Prostate Cancers

KP Porkka, EL Ogg, OR Saramaki, RL Vessella, H Pukkila, H Lahdesmaki, Wytske van Weerden, Monique Wolf, OP Kallioniemi, Guido Jenster, T Visakorpi

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MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate the expression of protein coding genes. In this study, we screened highly informative prostate cancer cell lines and xenografts (n = 42) for miRNA gene copy number and expression changes. The expression profiling showed distinction between cell lines and xenografts as well as between androgen sensitive and independent models. Only a few copy number alterations that were associated with expression changes were identified. Most importantly, the miR-15a-miR-16-1 locus was found to be homozygously deleted in two samples leading to the abolishment of miR-15a, but not miR-16, expression. miR-16 is also expressed from another genomic locus. Mutation screening of the miR-15a-miR-16-1 gene in the model systems as well as clinical samples (n = 50) revealed no additional mutations. In conclusion, our data indicate that putative tumor suppressors, miR-15a and miR-16-1, are homozygously deleted in a subset of prostate cancers, further suggesting that these miRNAs could be important in the development of prostate cancer. (C) 2011 Wiley-Liss, Inc.
Original languageUndefined/Unknown
Pages (from-to)499-509
Number of pages11
JournalGenes Chromosomes & Cancer
Issue number7
Publication statusPublished - 2011

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