The Rate of False Positive Sequence Matches of Peptides Profiled by MALDI MS and Identified by MS/MS

Marcel Stoop, RJAN Lamers, Peter Burgers, Peter Sillevis Smitt, Rogier Hintzen, Theo Luider

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)


In most MALDI peptide profiling cases, sequencing is required to identify peptides of interest, preferentially by using different mass spectrometry techniques. Using identical samples, we determined the number of false positive matches in sequence of peptide identification using different mass spectrometers. This paper demonstrates that the reliability of the identification phase greatly benefits from concerted MS-technologies and determines the influence of mass accuracy, signal-to-noise and statistical score on peptide identification.
Original languageUndefined/Unknown
Pages (from-to)4841-4847
Number of pages7
JournalJournal of Proteome Research
Issue number11
Publication statusPublished - 2008

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