The Receptor Binding Domain of the New Middle East Respiratory Syndrome Coronavirus Maps to a 231-Residue Region in the Spike Protein That Efficiently Elicits Neutralizing Antibodies

H Mou, Stalinraj Victor, FJM van Kuppeveld, PJM Rottier, Bart Haagmans, BJ (Berend Jan) Bosch

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Abstract

The spike (S) protein of the recently emerged human Middle East respiratory syndrome coronavirus (MERS-CoV) mediates infection by binding to the cellular receptor dipeptidyl peptidase 4 (DPP4). Here we mapped the receptor binding domain in the S protein to a 231-amino-acid fragment (residues 358 to 588) by evaluating the interaction of spike truncation variants with receptor-expressing cells and soluble DPP4. Antibodies to this domain-much less so those to the preceding N-terminal region-efficiently neutralize MERS-CoV infection.
Original languageUndefined/Unknown
Pages (from-to)9379-9383
Number of pages5
JournalJournal of Virology
Volume87
Issue number16
DOIs
Publication statusPublished - 2013

Research programs

  • EMC MM-04-27-01

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