The role of phosphatidylinositide-3-kinase in basal mitogen-activated protein kinase activity and cell survival

Henri H. Versteeg, Maaike W.A. Evertzen, Sander J.H. Van Deventer, Maikel P. Peppelenbosch*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Phosphatidylinositide-3-OH-kinase (PI 3-kinase) is an upstream activator of p42/p44 mitogen-activated protein kinase (MAPK), but the role of PI 3-kinase-dependent MAPK remains obscure. Here we demonstrate that in a variety of different cell types, PI 3-kinase inhibition results in an inhibition of MAPK in unstimulated cells but does not interfere with growth factor-, or TPA-induced MAPK activity. Furthermore, inhibition of either PI 3-kinase or MEK/MAPK results in cell death in serum-starved cells. We concluded that basal, but not induced MAPK activity is mediated by PI 3-kinase and that this PI 3-kinase-mediated MEK/MAPK activity is essential for cell survival in quiescent cells. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalFEBS Letters
Volume465
Issue number1
DOIs
Publication statusPublished - 7 Jan 2000
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by a grant from the Dutch Cancer Society (Koningin Wilhelmina Fonds) and a grant from the Dutch Heart Foundation (Nederlandse Hart Stichting).

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