The role of phosphatidylinositide-3-kinase in basal mitogen-activated protein kinase activity and cell survival

Henri H. Versteeg; Maaike W.A. Evertzen; Sander J.H. Van Deventer; Maikel P. Peppelenbosch

doi:10.1016/S0014-5793(99)01701-9

The role of phosphatidylinositide-3-kinase in basal mitogen-activated protein kinase activity and cell survival

Henri H. Versteeg, Maaike W.A. Evertzen, Sander J.H. Van Deventer, Maikel P. Peppelenbosch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

Phosphatidylinositide-3-OH-kinase (PI 3-kinase) is an upstream activator of p42/p44 mitogen-activated protein kinase (MAPK), but the role of PI 3-kinase-dependent MAPK remains obscure. Here we demonstrate that in a variety of different cell types, PI 3-kinase inhibition results in an inhibition of MAPK in unstimulated cells but does not interfere with growth factor-, or TPA-induced MAPK activity. Furthermore, inhibition of either PI 3-kinase or MEK/MAPK results in cell death in serum-starved cells. We concluded that basal, but not induced MAPK activity is mediated by PI 3-kinase and that this PI 3-kinase-mediated MEK/MAPK activity is essential for cell survival in quiescent cells. Copyright (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	69-73
Number of pages	5
Journal	FEBS Letters
Volume	465
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)01701-9
Publication status	Published - 7 Jan 2000
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by a grant from the Dutch Cancer Society (Koningin Wilhelmina Fonds) and a grant from the Dutch Heart Foundation (Nederlandse Hart Stichting).

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10.1016/S0014-5793(99)01701-9

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title = "The role of phosphatidylinositide-3-kinase in basal mitogen-activated protein kinase activity and cell survival",

abstract = "Phosphatidylinositide-3-OH-kinase (PI 3-kinase) is an upstream activator of p42/p44 mitogen-activated protein kinase (MAPK), but the role of PI 3-kinase-dependent MAPK remains obscure. Here we demonstrate that in a variety of different cell types, PI 3-kinase inhibition results in an inhibition of MAPK in unstimulated cells but does not interfere with growth factor-, or TPA-induced MAPK activity. Furthermore, inhibition of either PI 3-kinase or MEK/MAPK results in cell death in serum-starved cells. We concluded that basal, but not induced MAPK activity is mediated by PI 3-kinase and that this PI 3-kinase-mediated MEK/MAPK activity is essential for cell survival in quiescent cells. Copyright (C) 2000 Federation of European Biochemical Societies.",

author = "Versteeg, {Henri H.} and Evertzen, {Maaike W.A.} and {Van Deventer}, {Sander J.H.} and Peppelenbosch, {Maikel P.}",

note = "Funding Information: This study was supported by a grant from the Dutch Cancer Society (Koningin Wilhelmina Fonds) and a grant from the Dutch Heart Foundation (Nederlandse Hart Stichting).",

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AU - Versteeg, Henri H.

AU - Evertzen, Maaike W.A.

AU - Van Deventer, Sander J.H.

AU - Peppelenbosch, Maikel P.

N1 - Funding Information: This study was supported by a grant from the Dutch Cancer Society (Koningin Wilhelmina Fonds) and a grant from the Dutch Heart Foundation (Nederlandse Hart Stichting).

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N2 - Phosphatidylinositide-3-OH-kinase (PI 3-kinase) is an upstream activator of p42/p44 mitogen-activated protein kinase (MAPK), but the role of PI 3-kinase-dependent MAPK remains obscure. Here we demonstrate that in a variety of different cell types, PI 3-kinase inhibition results in an inhibition of MAPK in unstimulated cells but does not interfere with growth factor-, or TPA-induced MAPK activity. Furthermore, inhibition of either PI 3-kinase or MEK/MAPK results in cell death in serum-starved cells. We concluded that basal, but not induced MAPK activity is mediated by PI 3-kinase and that this PI 3-kinase-mediated MEK/MAPK activity is essential for cell survival in quiescent cells. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - Phosphatidylinositide-3-OH-kinase (PI 3-kinase) is an upstream activator of p42/p44 mitogen-activated protein kinase (MAPK), but the role of PI 3-kinase-dependent MAPK remains obscure. Here we demonstrate that in a variety of different cell types, PI 3-kinase inhibition results in an inhibition of MAPK in unstimulated cells but does not interfere with growth factor-, or TPA-induced MAPK activity. Furthermore, inhibition of either PI 3-kinase or MEK/MAPK results in cell death in serum-starved cells. We concluded that basal, but not induced MAPK activity is mediated by PI 3-kinase and that this PI 3-kinase-mediated MEK/MAPK activity is essential for cell survival in quiescent cells. Copyright (C) 2000 Federation of European Biochemical Societies.

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The role of phosphatidylinositide-3-kinase in basal mitogen-activated protein kinase activity and cell survival

Abstract

Bibliographical note

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