TY - JOUR
T1 - The role of receptor protein tyrosine phosphatase α in neuronal differentiation of embryonic stem cells
AU - Van Inzen, Wouter G.
AU - Peppelenbosch, Maikel P.
AU - Van Den Brand, Maria W.M.
AU - Tertoolen, Leon G.J.
AU - De Laat, Siegfried
PY - 1996/2/26
Y1 - 1996/2/26
N2 - In the present study, we have investigated the function of the receptor protein tyrosine phosphatase α (RPTPα) in the neuronal differentiation of E14-embryonic stem (E14-ES) cells. RNAase protection and western blot analysis revealed that E14-ES cells up regulate RPTPα expression upon neuronal differentiation with retinoic acid. Overexpression of RPTPα, by stable DNA transfection, and subsequent differentiation with retinoic acid, resulted in a temporally enhanced expression of the neuronal markers GAP-43 and NF-164. Electrophysiological experiments demonstrated that RPTPα overexpression also enhanced the development of neurotransmitter responses during differentiation. These results indicate that RPTPα plays an important role in the cascade of molecular events that lead to the formation of neurons.
AB - In the present study, we have investigated the function of the receptor protein tyrosine phosphatase α (RPTPα) in the neuronal differentiation of E14-embryonic stem (E14-ES) cells. RNAase protection and western blot analysis revealed that E14-ES cells up regulate RPTPα expression upon neuronal differentiation with retinoic acid. Overexpression of RPTPα, by stable DNA transfection, and subsequent differentiation with retinoic acid, resulted in a temporally enhanced expression of the neuronal markers GAP-43 and NF-164. Electrophysiological experiments demonstrated that RPTPα overexpression also enhanced the development of neurotransmitter responses during differentiation. These results indicate that RPTPα plays an important role in the cascade of molecular events that lead to the formation of neurons.
UR - http://www.scopus.com/inward/record.url?scp=0030022897&partnerID=8YFLogxK
U2 - 10.1016/0165-3806(95)00186-7
DO - 10.1016/0165-3806(95)00186-7
M3 - Article
C2 - 8852383
AN - SCOPUS:0030022897
SN - 0165-3806
VL - 91
SP - 304
EP - 307
JO - Developmental Brain Research
JF - Developmental Brain Research
IS - 2
ER -