TY - JOUR
T1 - The survival of epidemic and sporadic MRSA on human skin mimics is determined by both host and bacterial factors
AU - Baede, Valérie
AU - Voet, Michelle M.
AU - van der Reijden, Tanny J.K.
AU - van Wengen, Annelies
AU - Kreft, Deborah
AU - den Toom, Nicole
AU - Tavakol, Mehri
AU - Vos, Greet
AU - Nibbering, P. H.
AU - van Wamel, Willem
N1 - This work was part of the MACOTRA project, which was financially supported by JPIAMR 3 rd call, AMR Transmission dynamics and Dutch ZonMw (grant no. 547001006)
PY - 2022
Y1 - 2022
N2 - Bacterial survival on, and interactions with, human skin may explain the epidemiological success of MRSA strains. We evaluated the bacterial counts for 27 epidemic and 31 sporadic MRSA strains on 3D epidermal models based on N/TERT cells (NEMs) after 1, 2 and 8 days. In addition, the expression of antimicrobial peptides (hBD-2, RNase 7), inflammatory cytokines (IL-1β, IL-6) and chemokine IL-8 by NEMs was assessed using immunoassays and the expression of 43 S. aureus virulence factors was determined by a multiplex competitive Luminex assay. To explore donor variation, bacterial counts for five epidemic and seven sporadic MRSA strains were determined on 3D primary keratinocyte models (LEMs) from three human donors. Bacterial survival was comparable on NEMs between the two groups, but on LEMs, sporadic strains showed significantly lower survival numbers compared to epidemic strains. Both groups triggered the expression of immune factors. Upon interaction with NEMs, only the epidemic MRSA strains expressed pore-forming toxins, including alpha-hemolysin (Hla), gamma-hemolysin (HlgB), Panton-Valentine leucocidin (LukS) and LukED. Together, these data indicate that the outcome of the interaction between MRSA and human skin mimics, depends on the unique combination of bacterial strain and host factors.
AB - Bacterial survival on, and interactions with, human skin may explain the epidemiological success of MRSA strains. We evaluated the bacterial counts for 27 epidemic and 31 sporadic MRSA strains on 3D epidermal models based on N/TERT cells (NEMs) after 1, 2 and 8 days. In addition, the expression of antimicrobial peptides (hBD-2, RNase 7), inflammatory cytokines (IL-1β, IL-6) and chemokine IL-8 by NEMs was assessed using immunoassays and the expression of 43 S. aureus virulence factors was determined by a multiplex competitive Luminex assay. To explore donor variation, bacterial counts for five epidemic and seven sporadic MRSA strains were determined on 3D primary keratinocyte models (LEMs) from three human donors. Bacterial survival was comparable on NEMs between the two groups, but on LEMs, sporadic strains showed significantly lower survival numbers compared to epidemic strains. Both groups triggered the expression of immune factors. Upon interaction with NEMs, only the epidemic MRSA strains expressed pore-forming toxins, including alpha-hemolysin (Hla), gamma-hemolysin (HlgB), Panton-Valentine leucocidin (LukS) and LukED. Together, these data indicate that the outcome of the interaction between MRSA and human skin mimics, depends on the unique combination of bacterial strain and host factors.
UR - http://www.scopus.com/inward/record.url?scp=85143270705&partnerID=8YFLogxK
U2 - 10.1017/S0950268822001765
DO - 10.1017/S0950268822001765
M3 - Article
C2 - 36382385
SN - 0950-2688
VL - 150
SP - 751
EP - 762
JO - Epidemiology and Infection
JF - Epidemiology and Infection
M1 - e203
ER -
