TY - JOUR
T1 - The SW480 cell line as a model of resident and migrating colon cancer stem cells
AU - Verhagen, Mathijs P.
AU - Xu, Tong
AU - Stabile, Roberto
AU - Joosten, Rosalie
AU - Tucci, Francesco A.
AU - van Royen, Martin
AU - Trerotola, Marco
AU - Alberti, Saverio
AU - Sacchetti, Andrea
AU - Fodde, Riccardo
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024/9/20
Y1 - 2024/9/20
N2 - Intra-tumor heterogeneity, i.e., the presence of diverse cell types and subpopulations within tumors, presents a significant obstacle in cancer treatment due to its negative consequences for resistance to therapy and disease recurrence. However, the mechanisms that underlie intra-tumor heterogeneity and result in the plethora of different cancer cells within a single lesion remain poorly understood. Here, we leverage the SW480 cell line as a model system to investigate the molecular and functional diversity of colon cancer cells. Through a combination of fluorescence-activated cell sorting (FACS) analysis and transcriptomic profiling, we identified three distinct subpopulations, namely resident cancer stem cells (rCSCs), migratory CSCs (mCSCs), and high-relapse cells (HRCs). These subpopulations show varying Wnt signaling levels and gene expression profiles mirroring their stem-like and functional properties. Examination of publicly available spatial transcriptomic data confirms the presence of these subpopulations in patient-derived cancers and reveals their distinct spatial distribution relative to the tumor microenvironment.
AB - Intra-tumor heterogeneity, i.e., the presence of diverse cell types and subpopulations within tumors, presents a significant obstacle in cancer treatment due to its negative consequences for resistance to therapy and disease recurrence. However, the mechanisms that underlie intra-tumor heterogeneity and result in the plethora of different cancer cells within a single lesion remain poorly understood. Here, we leverage the SW480 cell line as a model system to investigate the molecular and functional diversity of colon cancer cells. Through a combination of fluorescence-activated cell sorting (FACS) analysis and transcriptomic profiling, we identified three distinct subpopulations, namely resident cancer stem cells (rCSCs), migratory CSCs (mCSCs), and high-relapse cells (HRCs). These subpopulations show varying Wnt signaling levels and gene expression profiles mirroring their stem-like and functional properties. Examination of publicly available spatial transcriptomic data confirms the presence of these subpopulations in patient-derived cancers and reveals their distinct spatial distribution relative to the tumor microenvironment.
UR - http://www.scopus.com/inward/record.url?scp=85201310767&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2024.110658
DO - 10.1016/j.isci.2024.110658
M3 - Article
C2 - 39246444
AN - SCOPUS:85201310767
VL - 27
JO - iScience
JF - iScience
IS - 9
M1 - 110658
ER -