The +TIP Navigator-1 is an actin-microtubule crosslinker that regulates axonal growth cone motility

Carlos Sánchez-Huertas*, Marion Bonhomme, Amandine Falco, Christine Fagotto-Kaufmann, Jeffrey van Haren, Freddy Jeanneteau, Niels Galjart, Anne Debant, Jérôme Boudeau

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)
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Abstract

Microtubule (MT) plus-end tracking proteins (+TIPs) are central players in the coordination between the MT and actin cytoskeletons in growth cones (GCs) during axon guidance. The +TIP Navigator-1 (NAV1) is expressed in the developing nervous system, yet its neuronal functions remain poorly elucidated. Here, we report that NAV1 controls the dynamics and motility of the axonal GCs of cortical neurons in an EB1-dependent manner and is required for axon turning toward a gradient of netrin-1. NAV1 accumulates in F-actin-rich domains of GCs and binds actin filaments in vitro. NAV1 can also bind MTs independently of EB1 in vitro and crosslinks nonpolymerizing MT plus ends to actin filaments in axonal GCs, preventing MT depolymerization in F-actin-rich areas. Together, our findings pinpoint NAV1 as a key player in the actin-MT crosstalk that promotes MT persistence at the GC periphery and regulates GC steering. Additionally, we present data assigning to NAV1 an important role in the radial migration of cortical projection neurons in vivo.

Original languageEnglish
Article numbere201905199
JournalJournal of Cell Biology
Volume219
Issue number9
DOIs
Publication statusPublished - 4 Jun 2020

Bibliographical note

© 2020 Sánchez-Huertas et al.

Research programs

  • EMC MGC-02-13-02
  • EMC OR-01

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